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Synthetic peptides QLVQFDCSPELFKKIKE and KAVQVVLRICYS, corresponding to amino acids 44-60 and 187-198 of Human PAIAS gamma.
Our Abpromise guarantee covers the use of ab13795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).|
Western blot analysis of PIAS gamma in Jurkat cell lysate using ab13795 at 2 ug/ml.
ab13795 at 1/50 staining human fibroblasts by ICC/IF. The cells were fixed with formaldehyde and blocked with serum prior to incubation with the antibody for 1 hour. An Alexa-Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary.
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