Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab58416)
Key features and details
- Rabbit polyclonal to E3 SUMO-protein ligase PIAS4/PIASy
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody
See all E3 SUMO-protein ligase PIAS4/PIASy primary antibodies -
Description
Rabbit polyclonal to E3 SUMO-protein ligase PIAS4/PIASy -
Host species
Rabbit -
Specificity
Detects endogenous levels of PAIAS gamma total protein. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human E3 SUMO-protein ligase PIAS4/PIASy.
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Positive control
- Jurkat cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/500 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
Target
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Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. -
Tissue specificity
Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes. -
Pathway
Protein modification; protein sumoylation. -
Sequence similarities
Belongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger. -
Domain
The LXXLL motif is a coregulator signature that is essential for transcriptional corepression. -
Post-translational
modificationsSumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activitation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate. -
Cellular localization
Nucleus > PML body. Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies. - Information by UniProt
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Database links
- Entrez Gene: 51588 Human
- Entrez Gene: 59004 Mouse
- Omim: 605989 Human
- SwissProt: Q8N2W9 Human
- SwissProt: Q3UGQ2 Mouse
- SwissProt: Q9JM05 Mouse
- Unigene: 105779 Human
- Unigene: 34428 Mouse
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Alternative names
- E3 SUMO protein ligase PIAS4 antibody
- E3 SUMO-protein ligase PIAS4 antibody
- FLJ12419 antibody
see all
Images
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All lanes : Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab58416) at 1/500 dilution
Lane 1 : Jurkat cell extract without immunizing peptide
Lane 2 : Jurkat cell extract with immunizing peptide
Predicted band size: 57 kDa
Observed band size: 57 kDa -
IHC image of ab58416 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58416, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab58416 has been referenced in 5 publications.
- Gao SS et al. TIP60 K430 SUMOylation attenuates its interaction with DNA-PKcs in S-phase cells: Facilitating homologous recombination and emerging target for cancer therapy. Sci Adv 6:eaba7822 (2020). PubMed: 32832608
- Fukuto A et al. SUMO modification system facilitates the exchange of histone variant H2A.Z-2 at DNA damage sites. Nucleus 9:87-94 (2018). PubMed: 29095668
- Yan Y et al. SUMOylation of AMPKa1 by PIAS4 specifically regulates mTORC1 signalling. Nat Commun 6:8979 (2015). PubMed: 26616021
- Fuhs SR & Insel PA Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects G-protein-coupled receptor desensitization. J Biol Chem 286:14830-41 (2011). WB ; Human . PubMed: 21362625
- Heo KS et al. PKC{zeta} mediates disturbed flow-induced endothelial apoptosis via p53 SUMOylation. J Cell Biol 193:867-84 (2011). PubMed: 21624955