• Product nameAnti-E3 SUMO-protein ligase PIAS4 antibody
    See all E3 SUMO-protein ligase PIAS4 primary antibodies
  • Description
    Rabbit polyclonal to E3 SUMO-protein ligase PIAS4
  • SpecificityDetects endogenous levels of PAIAS gamma total protein.
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from the C-terminus of human PAIAS gamma.

  • Positive control
    • Jurkat cell extract.


Associated products


Our Abpromise guarantee covers the use of ab58416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ELISA 1/20000.


  • FunctionFunctions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations.
  • Tissue specificityHighly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.
  • PathwayProtein modification; protein sumoylation.
  • Sequence similaritiesBelongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • DomainThe LXXLL motif is a coregulator signature that is essential for transcriptional corepression.
  • Post-translational
    Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activitation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.
  • Cellular localizationNucleus > PML body. Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 SUMO protein ligase PIAS4 antibody
    • E3 SUMO-protein ligase PIAS4 antibody
    • FLJ12419 antibody
    • MGC35296 antibody
    • PAIASgamma antibody
    • PIAS 4 antibody
    • PIAS gamma antibody
    • PIAS-gamma antibody
    • Pias4 antibody
    • PIAS4_HUMAN antibody
    • PIASG antibody
    • PIASgamma antibody
    • PIASy antibody
    • Protein inhibitor of activated STAT 4 antibody
    • Protein inhibitor of activated STAT protein 4 antibody
    • Protein inhibitor of activated STAT protein gamma antibody
    • Protein inhibitor of activated STAT protein PIASy antibody
    • Zinc finger MIZ type containing 6 antibody
    • ZMIZ 6 antibody
    • ZMIZ6 antibody
    see all

Anti-E3 SUMO-protein ligase PIAS4 antibody images

  • All lanes : Anti-E3 SUMO-protein ligase PIAS4 antibody (ab58416) at 1/500 dilution

    Lane 1 : Jurkat cell extract without immunizing peptide
    Lane 2 : Jurkat cell extract with immunizing peptide

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • IHC image of ab58416 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58416, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-E3 SUMO-protein ligase PIAS4 antibody (ab58416)

This product has been referenced in:
  • Yan Y  et al. SUMOylation of AMPKa1 by PIAS4 specifically regulates mTORC1 signalling. Nat Commun 6:8979 (2015). Read more (PubMed: 26616021) »
  • Fuhs SR & Insel PA Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects G-protein-coupled receptor desensitization. J Biol Chem 286:14830-41 (2011). WB ; Human . Read more (PubMed: 21362625) »

See all 3 Publications for this product

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