Anti-E3 SUMO-protein ligase PIAS4 antibody [PIA4] (ab61405)

Overview

  • Product nameAnti-E3 SUMO-protein ligase PIAS4 antibody [PIA4]
    See all E3 SUMO-protein ligase PIAS4 primary antibodies
  • Description
    Mouse monoclonal [PIA4] to E3 SUMO-protein ligase PIAS4
  • Tested applicationsSuitable for: WB, ICC, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide:

    EEGPRPKRRC PFQK

    , corresponding to amino acids 491-504 of Human PAIAS gamma

  • Positive control
    • WB: total cell extract of HEK-293T cells transfected with human PAIAS gamma

Properties

Applications

Our Abpromise guarantee covers the use of ab61405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa.
ICC Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • FunctionFunctions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations.
  • Tissue specificityHighly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.
  • PathwayProtein modification; protein sumoylation.
  • Sequence similaritiesBelongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • DomainThe LXXLL motif is a coregulator signature that is essential for transcriptional corepression.
  • Post-translational
    modifications
    Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activitation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.
  • Cellular localizationNucleus > PML body. Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 SUMO protein ligase PIAS4 antibody
    • E3 SUMO-protein ligase PIAS4 antibody
    • FLJ12419 antibody
    • MGC35296 antibody
    • PAIASgamma antibody
    • PIAS 4 antibody
    • PIAS gamma antibody
    • PIAS-gamma antibody
    • Pias4 antibody
    • PIAS4_HUMAN antibody
    • PIASG antibody
    • PIASgamma antibody
    • PIASy antibody
    • Protein inhibitor of activated STAT 4 antibody
    • Protein inhibitor of activated STAT protein 4 antibody
    • Protein inhibitor of activated STAT protein gamma antibody
    • Protein inhibitor of activated STAT protein PIASy antibody
    • Zinc finger MIZ type containing 6 antibody
    • ZMIZ 6 antibody
    • ZMIZ6 antibody
    see all

References for Anti-E3 SUMO-protein ligase PIAS4 antibody [PIA4] (ab61405)

ab61405 has not yet been referenced specifically in any publications.

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