Recombinant Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] (ab209263)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20241] to E3 ubiquitin-protein ligase MUL1
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241]
See all E3 ubiquitin-protein ligase MUL1 primary antibodies -
Description
Rabbit monoclonal [EPR20241] to E3 ubiquitin-protein ligase MUL1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293, HeLa, PC-3 and A431 whole cell lysates; Human fetal heart lysate. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20241 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/30.
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WB | (2) |
1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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Notes |
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IP
1/30. |
WB
1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
Target
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Function
Exhibits weak E3 ubiquitin-protein ligase activity, but preferentially acts as a SUMO E3 ligase at physiological concentrations. Plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. -
Tissue specificity
Widely expressed with highest levels in the heart, skeletal muscle, placenta, kidney and liver. Barely detectable in colon and thymus. -
Pathway
Protein modification; protein ubiquitination.
Protein modification; protein sumoylation. -
Sequence similarities
Contains 1 RING-type zinc finger. -
Domain
The zinc finger domain is required for E3 ligase activity. -
Cellular localization
Mitochondrion outer membrane. Peroxisome. Transported in mitochondrion-derived vesicles from the mitochondrion to the peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 79594 Human
- Omim: 612037 Human
- SwissProt: Q969V5 Human
- Unigene: 10101 Human
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Alternative names
- 0610009K11Rik antibody
- AV000801 antibody
- C1orf166 antibody
see all
Images
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All lanes : Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] (ab209263) at 1/1000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 4 : Human fetal heart tissue lysate
Lane 5 : Human thymus tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 15 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Negative control: Human thymus (PMID: 18591963).
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Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] (ab209263) at 1/1000 dilution + A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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E3 ubiquitin-protein ligase MUL1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab209263 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab209263 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate, 10 µg (Input).
Lane 2: ab209263 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209263 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (6)
ab209263 has been referenced in 6 publications.
- Mallard J et al. Chemotherapy impairs skeletal muscle mitochondrial homeostasis in early breast cancer patients. J Cachexia Sarcopenia Muscle 13:1896-1907 (2022). PubMed: 35373507
- Park M et al. The Role of Extracellular Vesicles in Optic Nerve Injury: Neuroprotection and Mitochondrial Homeostasis. Cells 11:N/A (2022). PubMed: 36496979
- Kim HJ et al. Crosstalk between HSPA5 arginylation and sequential ubiquitination leads to AKT degradation through autophagy flux. Autophagy 17:961-979 (2021). PubMed: 32164484
- Igarashi R et al. Gemcitabine induces Parkin-independent mitophagy through mitochondrial-resident E3 ligase MUL1-mediated stabilization of PINK1. Sci Rep 10:1465 (2020). PubMed: 32001742
- Kim SY et al. Non-Thermal Plasma Induces Antileukemic Effect Through mTOR Ubiquitination. Cells 9:N/A (2020). PubMed: 32131492
- Ordureau A et al. Global Landscape and Dynamics of Parkin and USP30-Dependent Ubiquitylomes in iNeurons during Mitophagic Signaling. Mol Cell 77:1124-1142.e10 (2020). PubMed: 32142685