Overview

  • Product nameAnti-E4F1 antibody
    See all E4F1 primary antibodies
  • Description
    Mouse polyclonal to E4F1
  • SpecificityThis antibody is reactive against mammalian transfected lysate.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    E4F1 (AAH80524.1, 1 a.a. ~ 784 a.a) full-length human protein.

  • Positive control
    • E4F1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 84 kDa).
    Detection Antibody Pair.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceE4F1 is a zinc finger transcription factor that inhibits cell proliferation. E4F1 interacts with Bmi1 in hematopoietic cells and is important for the regulation of stem cell proliferation.
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • E4F antibody
      • E4F transcription factor 1, isoform CRA_b antibody
      • P120E4F transcription factor antibody

    Anti-E4F1 antibody images

    • All lanes : Anti-E4F1 antibody (ab67875) at 1/500 dilution

      Lane 1 : E4F1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 84 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)

    References for Anti-E4F1 antibody (ab67875)

    ab67875 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab67875.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"