Overview

  • Product nameAnti-E4F1 antibody
    See all E4F1 primary antibodies
  • Description
    Rabbit polyclonal to E4F1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region between residue 275 and 325 of human E4F1

  • Positive control
    • HeLa, 293T, and MCF7 whole cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab70615 was affinity purified using an epitope specific to E4F1 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 84 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceE4F1 is a zinc finger transcription factor that inhibits cell proliferation. E4F1 interacts with Bmi1 in hematopoietic cells and is important for the regulation of stem cell proliferation.
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • E4F antibody
      • E4F transcription factor 1, isoform CRA_b antibody
      • P120E4F transcription factor antibody

    Anti-E4F1 antibody images

    • All lanes : Anti-E4F1 antibody (ab70615) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : 293T whole cell lysate
      Lane 3 : MCF7 whole cell lysate

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 84 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes
    • Detection of Human E4F1 by Western Blot of Immunprecipitate. ab70615 at 1µg/ml staining E4F1 in MCF7 whole cell lysates immunoprecipitated using ab70615 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 minutes

    References for Anti-E4F1 antibody (ab70615)

    ab70615 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"