The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000.
1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
FunctionTranscription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer.
Tissue specificityIn fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis.
Sequence similaritiesBelongs to the ETS family. Contains 1 ETS DNA-binding domain.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Immunocytochemistry/ Immunofluorescence - E74 like factor 1 antibody (ab64937)
This image shows A549 cells stained with ab64937 at 1/500 dilution; with and without the immunising peptide.
Western blot - E74 like factor 1 antibody (ab64937)
All lanes : Anti-E74 like factor 1 antibody (ab64937) at 1/500 dilution
Lane 1 : extracts from 293 cells (5-30µg total protein) Lane 2 : extracts from HuvEc cells (5-30µg total protein) Lane 3 : extracts from HuvEc cells (5-30µg total protein) and 5-10µg of the immunising peptide.
Predicted band size : 67 kDa Observed band size : 67 kDa
References for Anti-E74 like factor 1 antibody (ab64937)
has not yet been referenced specifically in any publications.
Publishing research using ab64937? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"