The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000.
1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer.
In fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis.
Belongs to the ETS family. Contains 1 ETS DNA-binding domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Immunocytochemistry/ Immunofluorescence - E74 like factor 1 antibody (ab64937)
This image shows A549 cells stained with ab64937 at 1/500 dilution; with and without the immunising peptide.
Western blot - E74 like factor 1 antibody (ab64937)
All lanes : Anti-E74 like factor 1 antibody (ab64937) at 1/500 dilution
Lane 1 : extracts from 293 cells (5-30µg total protein) Lane 2 : extracts from HuvEc cells (5-30µg total protein) Lane 3 : extracts from HuvEc cells (5-30µg total protein) and 5-10µg of the immunising peptide.
Predicted band size: 67 kDa Observed band size: 67 kDa