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Western blot - (ab37969)
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Immunocytochemistry/ Immunofluorescence - eEF1A1 antibody (ab37969)
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Western blot - eEF1A1 antibody (ab37969)
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Product Name
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eEF1A1 antibody
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See all eEF1A1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to eEF1A1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human eEF1A1. (Peptide available as ab37968.)
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Cat, Chk, Chmp, Cow, Dog, Hm, Hrs, Mcq, Pig, Rb, Shp
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following
Human Whole Cell Lysates
HeLa
Jurkat
A431
HEK 293
HepG2
MCF-7
SHSY-5Y
U2OS
Mouse Whole Cell Lysates
NIH 3T3
MEF1
Mouse Tissue Lysates
Brain
Liver
Heart
Kidney
Pancreas
Testis
Skeletal muscle
Spinal Cord
Ovary
Rat Whole Cell Lysate
PC12
Rat Tissue Lysate
Brain
Liver
Heart
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Cellular localization
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Cytoplasmic
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> tRNAs
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Relevance
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eEF1A1 is an isoform of the alpha subunit of the elongation factor 1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. It promotes the GTP dependent binding of aminoacyl tRNA to the A site of ribosomes during protein biosynthesis. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome (referenced from entrez gene and swissprot).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this eEF1A1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for eEF1A1 antibody images, references, products related to ab37969 and other tools.
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eEF1A1 antibody images:
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 Immunocytochemistry/ Immunofluorescence - eEF1A1 antibody (ab37969)
ICC/IF image of ab37969 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37969, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Western blot - eEF1A1 antibody (ab37969)
All lanes : eEF1A1 antibody (ab37969) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 5 : Heart (Mouse) Tissue Lysate
Lane 6 : Kidney (Mouse) Tissue Lysate
Lane 7 : Pancreas (Mouse) Tissue Lysate (ab29363)
Lane 8 : Testis (Mouse) Tissue Lysate
Lane 9 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)
Lane 10 : Spinal Cord (Mouse) Tissue Lysate
Lane 11 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 12 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 13 : Brain (Rat) Tissue Lysate - normal tissue
Lane 14 : Liver (Rat) Tissue Lysate
Lane 15 : Heart (Rat) Tissue Lysate
Lane 16 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
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References for eEF1A1 antibody (ab37969)
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This product has been used in: (two most recent references)
Vessey JP et al. Mammalian Pumilio 2 regulates dendrite morphogenesis and synaptic function. Proc Natl Acad Sci U S A 107:3222-7 (2010). WB. PubMed: 20133610
Andersen KM et al. Thioredoxin Txnl1/TRP32 is a redox-active cofactor of the 26 S proteasome. J Biol Chem 284:15246-54 (2009). WB; Human. PubMed: 19349277
If you publish research using ab37969 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to eEF1A1
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eEF1A1 antibody - more information
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Customer reviews (feedback) regarding eEF1A1 antibody |
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eEF1A1 antibody - related products:
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eEF1A1 antibody - other tools:
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