Specific protocols
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To our knowledge, customised protocols are not required for this product.
Please try the standard protocols listed below and let us know how you get on. |
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General protocols
Useful resources:Western blotting (WB) protocols:Immunohistochemistry (IHC) / Immunocytochemistry (ICC) protocols:Chromatin Immunoprecipitation (ChIP) protocols:Dot blot protocols:ELISA protocols:ELISPOT protocols: Flow cytometry / FACS protocols:Immunoprecipitation (IP) protocols: |
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Anti-eEF1A1 antibody (ab37969)
All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa
Immunocytochemistry/ Immunofluorescence - eEF1A1 antibody (ab37969)
ICC/IF image of ab37969 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37969, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Western blot - eEF1A1 antibody (ab37969)
All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 5 : Heart (Mouse) Tissue Lysate
Lane 6 : Kidney (Mouse) Tissue Lysate
Lane 7 : Pancreas (Mouse) Tissue Lysate (ab29363)
Lane 8 : Testis (Mouse) Tissue Lysate
Lane 9 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)
Lane 10 : Spinal Cord (Mouse) Tissue Lysate
Lane 11 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 12 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 13 : Brain (Rat) Tissue Lysate - normal tissue
Lane 14 : Liver (Rat) Tissue Lysate
Lane 15 : Heart (Rat) Tissue Lysate
Lane 16 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-eEF1A1 antibody (ab37969)
All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 : eEF1A1 protein (Tagged) (ab81792)
Lane 2 : EEF1A2 protein (T7 Tag) (ab40317)
Lysates/proteins at 0.1 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa
Exposure time : 3 minutes
eEF1A1 antibody for ICC/IF in Human (37969)
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