Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNAs
|
ab37968 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab37969 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
|
|||||||||
Thank you for the information. We will take a refund, and I will find out the order number for you and get back to you. Can you please tell me how you know the antibody is cross-reacting with eEF1A3, as it will help us to interpret our results if we can run a positive and negative control for eEF1A3. Many Thanks, |
|||||||||
ANSWER: |
Thank you for your reply, and I apologize again for this error.
I found your order *** from October 2008, and I have asked our finance department to issue credit note #***. The credit note may be used in one of the following ways:
(1) Redeemed against the original invoice if this hasn't already been paid. (2) Held on the account for use against a future order. (3) A full refund can be offered where no other invoices are outstanding.
To receive a refund, please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.
The cross-reactivity with eEF1A2 and eEF1A3 was predicted based on sequence homology and then confirmed by Western blot against the recombinant proteins. I hope this information is helpful in interpreting your results, but please let me know if you need any further information and I'll be happy to help. |
||||||||
|
|||||||||
Thanks for your email. Would you be able to let me know our PO no. for item ab37969 and I could then pass your email on to the end user of this product?
Many thanks. |
|||||||||
ANSWER: |
Thank you for your reply.
The PO# on this order is 1244827. I appreciate you forwarding the email to the end user.
Please let me know if you need any more information from me. |
||||||||
|
|||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Lane 3 :
Lane 4 :
Lane 5 :
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa
ICC/IF image of ab37969 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37969, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 :
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 5 : Heart (Mouse) Tissue Lysate
Lane 6 : Kidney (Mouse) Tissue Lysate
Lane 7 :
Lane 8 : Testis (Mouse) Tissue Lysate
Lane 9 :
Lane 10 : Spinal Cord (Mouse) Tissue Lysate
Lane 11 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 12 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 13 : Brain (Rat) Tissue Lysate - normal tissue
Lane 14 : Liver (Rat) Tissue Lysate
Lane 15 : Heart (Rat) Tissue Lysate
Lane 16 :
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 :
Lane 2 :
Lysates/proteins at 0.1 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa
Exposure time : 3 minutes
1
Call 01223 696 000 or contact us