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eEF1A1 protein (Tagged) (ab81792)

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Overview

Product name

eEF1A1 protein (Tagged)
See all eEF1A1 products (2) ...

Protein description

Recombinant full length human eEF1A1 with proprietary tag (SwissProt P68104).

Expression host

E. coli

Properties

Purity

Ion Exchange Chromatography

Purification notes

ab81792 is purified by an affinity column in combination with FPLC chromatography. Purified recombinant protein is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity. 1 unit equals 1 nanogram of purified protein.

Form

Liquid

Storage instructions

Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8

Concentration

Concentration information loading...

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNAs

Applications

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Our Abpromise guarantee covers the use of ab81792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Protein info

Function

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Tissue specificity

Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.

Sequence similarities

Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Post-translational
modifications

ISGylated.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP68104 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

References for eEF1A1 protein (Tagged) (ab81792)

ab81792 has not yet been referenced specifically in any publications.

Publishing research using ab81792? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"