|
|
|
|
Western blot - eIF1A antibody (ab38976)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence - eIF1A antibody (ab38976)
(enlarge)
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF1A antibody (ab38976)
(enlarge)
|
|
|
|
|
Product Name
|
eIF1A antibody
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to eIF1A
|
|
Immunogen
|
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human eIF1A. (Peptide available as ab39512.)
|
|
Reacts with
|
Hu
|
|
Predicted to react
(species key)
|
Ms, Rat, Chk, Chmp, Cow, Dm, Rb, Xl, Zfsh
(Due to sequence homology)
|
|
Tested applications
(see key)
|
ICC/IF, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
This antibody gave a positive signal in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line)
Jurkat (Human T cell lymphoblast-like cell line)
A431 (Human epithelial carcinoma cell line)
|
|
Cellular localization
|
Cytoplasmic and Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
|
|
Relevance
|
eIF1A is an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA (referenced from entrez gene).
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.800 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this eIF1A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for eIF1A antibody images, references, products related to ab38976 and other tools.
|
| |
eIF1A antibody images:
|
|
|
| |
 Immunocytochemistry/ Immunofluorescence - eIF1A antibody (ab38976)
ICC/IF image of ab38976 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38976, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF1A antibody (ab38976)
IHC image of eIF1A1 staining in human skin FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38976, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
|
| |
| |
|
Search PubMed (MEDLINE) for references to eIF1A
|
| |
eIF1A antibody - more information
|
Customer reviews (feedback) regarding eIF1A antibody |
Customer FAQs regarding eIF1A antibody |
Protocols for eIF1A antibody |
Price and availability of products related to eIF1A antibody |
| |
eIF1A antibody - related products:
|
|
|
| |
eIF1A antibody - other tools:
|
Contact Abcam with a Technical Enquiry about eIF1A antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
