eIF2 alpha (phospho S51) antibody [E90] (ab32157)

Product Name 

eIF2 alpha (phospho S51) antibody [E90]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [E90] to eIF2 alpha (phospho S51)

Immunogen 

A synthetic phospho-peptide corresponding to residues surrounding Ser51 of human eIF-2a.

Reacts with 

Hu, Ms, Rat, AGMk, Dm

Predicted to react 
(species key)

Cow, Pig
(Due to sequence homology)

Specificity 

The antibody only detects eIF-2a phosphorylated on Serine 51.

Tested applications 
(see key)

Dot, ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Dot: 1/500.
ICC: 1/100 - 1/250.
IHC-P: 1/50 - 1/100.
WB: 1/500. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

Is unsuitable for FACS or IP.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

PC12 cell lysate, human liver carcinoma.

Research areas 

Chromatin and Nuclear Signaling >> RNAi >> Eukaryotic Initiation factors (eIF's)
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

Relevance 

eIF2 alpha is a 36 kDa protein which is ubiquitously expressed in many cell types. The eIF2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. In mammalian cells, eIF2 alpha is phosphorylated at serine 51 (human EIF2 alpha, the equivalent residue in mouse is serine 52) by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF2 alpha blocks the GDP-GTP exchange activity of eIF2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF2 alpha is an important regulatory process in protein synthesis.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

E90

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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References for eIF2 alpha (phospho S51) antibody [E90] (ab32157)

  This product has been used in: (two most recent references)

Duffy C et al. VP22 of herpes simplex virus 1 promotes protein synthesis at late times in infection and accumulation of a subset of viral mRNAs at early times in infection. J Virol 83:1009-17 (2009). WB; African Green Monkey. PubMed: 18987147

Farny NG et al. Metazoan stress granule assembly is mediated by P-eIF2alpha-dependent and -independent mechanisms. RNA 15:1814-21 (2009). WB; Fruit fly (Drosophila melanogaster). PubMed: 19661161

See all 4 publication references for this product.


If you publish research using ab32157 please let us know so that we can cite the reference on this datasheet.

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