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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-EIF2S1 (phospho S51) antibody
See all EIF2S1 products (14) ...
Description
Rabbit polyclonal to EIF2S1 (phospho S51)
Specificity
This antibody is specific for the phosphorylated form of eIF-2 alpha. It recognizes an epitope that includes phosphorylated Serine 52. The antibody has been cross adsorbed to remove activity to the non-phosphorylated form.
Tested applications
WB, IP, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Dog, Human, Pig, Monkey
Immunogen
A 13 residue phosphorylated synthetic peptide (Rat) (Internal sequence).
Positive control
HeLa cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab19288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IP: Use a concentration of 12.5 µg/ml
ICC: Use at an assay dependent dilution.
Target
Function
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Sequence similarities
Belongs to the eIF-2-alpha family.
Contains 1 S1 motif domain.
Post-translational
modifications
Substrate for at least 4 kinases: EIF2AK3/PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. In case of infection by vaccinia virus or rotavirus A, eIF2S1 phosphorylation state is modulated.
Cellular localization
Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules.
Target information above from: UniProt accessionP05198
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EIF 2 alpha antibody
- EIF 2 antibody
- EIF 2A antibody
see all
Database links
- Entrez Gene: 480361 Dog
- Entrez Gene: 1965 Human
- Entrez Gene: 13665 Mouse
- Entrez Gene: 54318 Rat
- Omim: 603907 Human
- SwissProt: P05198 Human
- SwissProt: Q6ZWX6 Mouse
- SwissProt: P68101 Rat
see all
References for Anti-EIF2S1 (phospho S51) antibody (ab19288)
This product has been referenced in:
- Eley HLet al. Increased expression of phosphorylated forms of RNA-dependent protein kinase and eukaryotic initiation factor 2alpha may signal skeletal muscle atrophy in weight-losing cancer patients. Br J Cancer 98:443-9 (2008). WB; Human.Read more (PubMed: 18087277) »
- Eley HLet al. Signaling pathways initiated by beta-hydroxy-beta-methylbutyrate to attenuate the depression of protein synthesis in skeletal muscle in response to cachectic stimuli. Am J Physiol Endocrinol Metab 293:E923-31 (2007). WB; Mouse.Read more (PubMed: 17609254) »
See all 3 publications for this product
Publishing research using ab19288? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"