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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-EIF2S1 (phospho S51) antibody
See all EIF2S1 products (14) ...
Description
Rabbit polyclonal to EIF2S1 (phospho S51)
Specificity
This antibody detects eIF2a only when phosphorylated at serine 51.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthesized phosphopeptide derived from human EIF2S1 around the phosphorylation site of serine 51 (E-L-SP-R-R).
Positive control
K562 cell extract.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - EIF2S1 (phospho S51) antibody (ab47769)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Sequence similarities
Belongs to the eIF-2-alpha family.
Contains 1 S1 motif domain.
Post-translational
modifications
Substrate for at least 4 kinases: EIF2AK3/PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. In case of infection by vaccinia virus or rotavirus A, eIF2S1 phosphorylation state is modulated.
Cellular localization
Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules.
Target information above from: UniProt accessionP05198
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EIF 2 alpha antibody
- EIF 2 antibody
- EIF 2A antibody
see all
Database links
- Entrez Gene: 1965 Human
- Entrez Gene: 13665 Mouse
- Entrez Gene: 54318 Rat
- Omim: 603907 Human
- SwissProt: P05198 Human
- SwissProt: Q6ZWX6 Mouse
- SwissProt: P68101 Rat
- Unigene: 151777 Human
see all
Anti-EIF2S1 (phospho S51) antibody images:
Western blot - EIF2S1 (phospho S51) antibody (ab47769)
All lanes : Anti-EIF2S1 (phospho S51) antibody (ab47769) at 1/500 dilution
Lane 1 : K562 cell extract, untreated.
Lane 2 : K562 cell extract treated with IFN alpha (1000U/ml, 18 hours)
Predicted band size : 36 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Typically 5-30ug of total protein was loaded per lane of the gel.
References for Anti-EIF2S1 (phospho S51) antibody (ab47769)
ab47769 has not yet been referenced specifically in any publications.
Publishing research using ab47769? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"