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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Anti-eIF2B epsilon antibody (ab32713)
Product partner
Related products
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Proteins and Peptides
ab32712
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab32713? Please let us know so that we can cite the reference in this datasheet
ab32713 has been referenced in 3 publications.
- Toth MJ et al. Chronic heart failure reduces Akt phosphorylation in human skeletal muscle: relationship to muscle size and function. J Appl Physiol 110:892-900 (2011). PubMed: 21193562
- Glover EI et al. Resistance exercise decreases eIF2B{epsilon} phosphorylation and potentiates the feeding-induced stimulation of p70S6k1 and rpS6 in young men. Am J Physiol Regul Integr Comp Physiol : (2008). PubMed: 18565837
- Yung HW et al. Evidence of placental translation inhibition and endoplasmic reticulum stress in the etiology of human intrauterine growth restriction. Am J Pathol 173:451-62 (2008). WB; Human. PubMed: 18583310
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - eIF2B epsilon antibody (ab32713)
All lanes : Anti-eIF2B epsilon antibody (ab32713) at 1/500 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Lane 3 :
Lane 4 :
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 80 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa (possible cleavage fragment),73 kDa (possible cleavage fragment).
Western blot - eIF2B epsilon antibody (ab32713)
All lanes : Anti-eIF2B epsilon antibody (ab32713) at 1/250 dilution
Lane 1 :
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 6 : Brain (Rat) Tissue Lysate - normal tissue
Lane 7 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 80 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa (possible cleavage fragment).
Immunocytochemistry/ Immunofluorescence - eIF2B epsilon antibody (ab32713)
ICC/IF image of ab32713 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32713, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunocytochemistry/ Immunofluorescence-eIF2B epsilon antibody(ab32713)
ICC/IF image of ab32713 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32713, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
eIF2B epsilon antibody for WB in Human (32713)
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