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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-eIF2B epsilon antibody
See all eIF2B epsilon products (5) ...
Description
Rabbit polyclonal to eIF2B epsilon
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human eIF2B epsilon.
(Peptide available as ab32712.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK 293 (Human embryonic kidney cell line) NIH 3T3 (Mouse embryonic fibroblast cell line) MEF1 (Mouse embryonic fibroblast cell line) PC12 (Rat adrenal pheochromocytoma cell line) Brain (Mouse and Rat)Tissue lysates Liver (Mouse and Rat)Tissue lysates
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
Involvement in disease
Defects in EIF2B5 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy.
Sequence similarities
Belongs to the eIF-2B gamma/epsilon subunits family.
Contains 1 W2 domain.
Target information above from: UniProt accessionQ13144
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CACH antibody
- CLE antibody
- EI2BE_HUMAN antibody
see all
Database links
- Entrez Gene: 8893 Human
- Entrez Gene: 224045 Mouse
- Entrez Gene: 192234 Rat
- Omim: 603945 Human
- SwissProt: Q13144 Human
- SwissProt: Q8CHW4 Mouse
- SwissProt: Q64350 Rat
- Unigene: 283551 Human
see all
Anti-eIF2B epsilon antibody images:
Western blot - eIF2B epsilon antibody (ab32713)
All lanes : Anti-eIF2B epsilon antibody (ab32713) at 1/500 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 80 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa (possible cleavage fragment),73 kDa (possible cleavage fragment).
Western blot - eIF2B epsilon antibody (ab32713)
All lanes : Anti-eIF2B epsilon antibody (ab32713) at 1/250 dilution
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 6 : Brain (Rat) Tissue Lysate - normal tissue
Lane 7 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 80 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa (possible cleavage fragment).
Immunocytochemistry/ Immunofluorescence - eIF2B epsilon antibody (ab32713)
ICC/IF image of ab32713 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32713, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunocytochemistry/ Immunofluorescence-eIF2B epsilon antibody(ab32713)
ICC/IF image of ab32713 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32713, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-eIF2B epsilon antibody (ab32713)
This product has been referenced in:
- Toth MJet al. Chronic heart failure reduces Akt phosphorylation in human skeletal muscle: relationship to muscle size and function. J Appl Physiol 110:892-900 (2011).Read more (PubMed: 21193562) »
- Glover EIet al. Resistance exercise decreases eIF2B{epsilon} phosphorylation and potentiates the feeding-induced stimulation of p70S6k1 and rpS6 in young men. Am J Physiol Regul Integr Comp Physiol : (2008).Read more (PubMed: 18565837) »
See all 3 publications for this product
Publishing research using ab32713? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"