Anti-EIF2B5 antibody (ab91565)
Key features and details
- Rabbit polyclonal to EIF2B5
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EIF2B5 antibody
See all EIF2B5 primary antibodies -
Description
Rabbit polyclonal to EIF2B5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human EIF2B5 aa 400-450.
Database link: NP_003898.2 -
Positive control
- HeLa and 293T whole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab91565 was affinity purified using an epitope specific to EIF2B5 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 80 kDa.
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IP |
Use at 5-15 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 80 kDa. |
IP
Use at 5-15 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. -
Involvement in disease
Defects in EIF2B5 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy. -
Sequence similarities
Belongs to the eIF-2B gamma/epsilon subunits family.
Contains 1 W2 domain. - Information by UniProt
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Database links
- Entrez Gene: 8893 Human
- Omim: 603945 Human
- SwissProt: Q13144 Human
- Unigene: 283551 Human
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Alternative names
- CACH antibody
- CLE antibody
- EI2BE_HUMAN antibody
see all
Images
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All lanes : Anti-EIF2B5 antibody (ab91565) at 0.1 µg/ml
Lanes 1 & 4 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 80 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling EIF2B5 with ab91565 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of EIF2B5 by Western Blot of Immunprecipitate.
ab91565, at 1 µg/ml, staining EIF2B5 in HeLa whole cell lysate immunoprecipitated using ab91565 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab91565 has not yet been referenced specifically in any publications.