|
|
|
|
Western blot - eIF2B epsilon (phospho S539) antibody (ab4775)
(enlarge)
|
|
|
|
|
Product Name
|
eIF2B epsilon (phospho S539) antibody
|
| |
See all eIF2B epsilon antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to eIF2B epsilon (phospho S539)
|
|
Immunogen
|
Synthetic peptide (Rat) derived from the region of rat eIF-2B epsilon that contains serine 535 (corresponding to serine 539 in the human sequence).
|
|
Reacts with
(species key)
|
Hu, Rat
|
|
Tested applications
(see key)
|
WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
WB: Use at a concentration of 0.1 - 1.0 µg/ml. Detects a band of approximately 88 kDa (predicted molecular weight: 82 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Recombinant eIF-2B epsilon treated with GSK 3 beta and lambda phosphatase, CHO-T cells transfected with human insulin receptor (IR) +/- insulin.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Chromatin and Nuclear Signaling >> RNAi >> Eukaryotic Initiation factors (eIF's)
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
|
|
Relevance
|
Eukaryotic Initiation Factor 2B (eIF-2B) plays a key role by acting as a guanine nucleotide exchange factor in the initiation of mRNA translation in eukaryotic cells. The activity of eIF-2B is regulated by a wide array of conditions. Stress inhibits its action, while stimulation of protein synthesis results in activation. eIF-2B epsilon, an 82 kDa subunit of eIF-2B, is phosphorylated at serine 539 by glycogen synthase kinase 3 (GSK 3), which leads to inhibition of its guanine nucleotide exchange activity.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.05% Sodium Azide
Constituents: PBS, 1.0mg/ml BSA (IgG, protease free). pH 7.3
Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively pre-adsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated eukaryotic initiation factor 2B epsilon (eIF-2B epsilon), and (ii) a generic serine phosphorylated peptide to remove antibody that is reactive with phospho-serine, irrespective of the sequence. The final product is generated by affinity chromatography using an eIF-2B epsilon-derived peptide that is phosphorylated at serine 539.
|
|
Form
|
Liquid
|
|
Concentration
|
0.500 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this eIF2B epsilon (phospho S539) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for eIF2B epsilon (phospho S539) antibody images, references, products related to ab4775 and other tools.
|
| |
eIF2B epsilon (phospho S539) antibody images:
|
 Western blot - eIF2B epsilon (phospho S539) antibody (ab4775)
Predicted band size : 82 kDa
Peptide Competition: Recombinant eIF-2B episilon treated with lambda phosphatase (1) or treated with GSK-3 beta (lanes 2-5) and added to background extracts were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4775 antibody, following prior incubation with: no peptide (1, 2), the nonphosphopeptide corresponding to the immunogen (3), a generic phophoserine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4775 blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show the up-regulation of the ab4775 phospho signal upon stimulation with GSK-3 beta.
|
| |
References for eIF2B epsilon (phospho S539) antibody (ab4775)
|
|
This product has been used in: (two most recent references)
Yung HW et al. Evidence of placental translation inhibition and endoplasmic reticulum stress in the etiology of human intrauterine growth restriction. Am J Pathol 173:451-62 (2008). WB; Human. PubMed: 18583310
Vary TC et al. Phosphorylation of eukaryotic initiation factor eIF2Bepsilon in skeletal muscle during sepsis. Am J Physiol Endocrinol Metab 283:E1032-9 (2002). PubMed: 12376332
If you publish research using ab4775 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to eIF2B epsilon
|
| |
eIF2B epsilon (phospho S539) antibody - more information
|
Customer reviews (feedback) regarding eIF2B epsilon (phospho S539) antibody |
Customer FAQs regarding eIF2B epsilon (phospho S539) antibody |
Protocols for eIF2B epsilon (phospho S539) antibody |
Price and availability of products related to eIF2B epsilon (phospho S539) antibody |
| |
eIF2B epsilon (phospho S539) antibody - related products:
|
|
|
| |
eIF2B epsilon (phospho S539) antibody - other tools:
|
Contact Abcam with a Technical Enquiry about eIF2B epsilon (phospho S539) antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
