![Western blot - eIF3K antibody [2313C2a] (ab50736) image](#ab50736.bmp)
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Product name
Anti-eIF3K antibody [2313C2a]
See all eIF3K products (6) ...
Description
Mouse monoclonal [2313C2a] to eIF3K
Tested applications
WB, Dot Blotmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant human EIF3S12
Positive control
Recombinant human EIF3S12
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM KH2PO4, 8.0mM Na2HPO4, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab50736 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Clonality
Monoclonal
Clone number
2313C2a
Isotype
IgG2b
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Western blot - eIF3K antibody [2313C2a] (ab50736)
![Western blot - eIF3K antibody [2313C2a] (ab50736) image](/ps/datasheet/Images/50/ab50736/ab50736.bmp)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab50736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa.
Dot: Use at an assay dependent dilution.
Target
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Tissue specificity
Ubiquitous, with the highest levels of expression in brain, testis and kidney.
Sequence similarities
Belongs to the eIF-3 subunit K family.
Cellular localization
Nucleus. Cytoplasm.
Target information above from: UniProt accessionQ9UBQ5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ARG134 antibody
- eIF 3 p25 antibody
- eIF 3 p28 antibody
see all
Database links
- Entrez Gene: 27335 Human
- Omim: 609596 Human
- SwissProt: Q9UBQ5 Human
- Unigene: 314359 Human
Anti-eIF3K antibody [2313C2a] images:
Western blot - eIF3K antibody [2313C2a] (ab50736)
Anti-eIF3K antibody [2313C2a] (ab50736) + immunized recombinant protein
Predicted band size : 25 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
References for Anti-eIF3K antibody [2313C2a] (ab50736)
This product has been referenced in:
- Cohen AA et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB; Human.Read more (PubMed: 19023046) »
See 1 publication for this product
Publishing research using ab50736? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"