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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-eIF4B antibody
See all eIF4B products (7) ...
Description
Rabbit polyclonal to eIF4B
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human eIF4B.
(Peptide available as ab38358.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK293 (Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) SHSY-5Y (Human neuroblastoma cell line)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
ICC/IF: Use a concentration of 1 µg/ml
Target
Relevance
eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).
Cellular localization
Cytoplasmic
Alternative names
- EIF 4B antibody
- EIF-4B antibody
- Eukaryotic initiation factor 4B antibody
see all
Database links
- Entrez Gene: 505850 Cow
- Entrez Gene: 1975 Human
- Entrez Gene: 75705 Mouse
- Entrez Gene: 300253 Rat
- Entrez Gene: 553214 Zebrafish
- Omim: 603928 Human
- SwissProt: Q3MHP6 Cow
- SwissProt: P23588 Human
see all
Anti-eIF4B antibody images:
Western blot - Anti-eIF4B antibody (ab38359)
All lanes : Anti-eIF4B antibody (ab38359) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 69 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - eIF4B antibody (ab38359)
ICC/IF image of ab38359 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38359, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-eIF4B antibody (ab38359)
ab38359 has not yet been referenced specifically in any publications.
Publishing research using ab38359? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"