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All lanes : Anti-eIF4B antibody (ab71057) at 1/50 dilution
Lane 1 : 293 cell lysate, non transfected
Lane 2 : 293 cell lysate, transiently transfected with the eIF4B gene
Lysates/proteins at 2 µg per lane.
Observed band size : 79 kDa (why is the actual band size different from the predicted?)
ab71057, at a dilution of 1/50, staining eIF4B in formalin fixed, paraffin embedded human breast carcinoma tissue (peroxidase conjugated secondary antibody, DAB staining).
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