Anti-eIF4B antibody (ab78916)

I am interested in purchasing an anti-eIF4B antibody from you. It appears you have three such antibodies ab38359, ab66780, ab78916. I would like to use the antibody in western blot, immunofluorescence and IHC in human tissue and cell lines. Ideally I would like one antibody that could work in all three applications. ab78916 seems to be the most widely validated antibody in that images for these applications are available on your website. However, the IF image for ab38359 is much more convincing. Could you tell me the reason why you are marketing all three antibodies? Could you give me advice on which one to try?   Thanks very much for your help

ANSWER:

Thank you very much for contacting us regarding our eIF4B antibodies.

We sell these three particular antibodies because they have been derived from different areas of human eIF4B. Ab78916 is derived from human eIF4B around the phosphorylation site of serine 422 (T-G-SP-E-S). Ab66780 corresponding to a region within C terminal amino acids 505-554 and ab38359 derived from within residues 300 - 400 of Human eIF4B.

If an antibody has not been tested in an assay or one a species with >85% homology, I can offer a discount off a future purchase if you buy that now, test it and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

If you are not interested in creating an abreview, I would suggest using ab78916 as it has been tested for WB, IHC and ICC-IF. Our Abpromise ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund. The Abcam Abpromise® guarantee: • 100% Scientific and Customer Support for any product you buy from Abcam or one of our authorized distributors. • We guarantee our products work in the tested species and applications stated on the datasheet. • We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase. • We investigate any quality concerns raised by customers to ensure our catalog contains products that perform to the highest standards. Please note these conditions to our Abpromise®: • Protocol information must be provided in order for the claim to be validated. • Antibodies tested in recombinant samples only are not guaranteed for use on endogenous samples. • “Predicted to react” information on the datasheets is provided for reference only and these species are not guaranteed. • Fast Track antibodies are covered for ELISA against the immunizing peptide only.

I hope this information is helpful, but please do not hesitate to contact us with further questions.