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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-eIF4B antibody
See all eIF4B products (7) ...
Description
Rabbit polyclonal to eIF4B
Tested applications
WB, ELISA, IHC-P, ICC/IF, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic non-phosphopeptide derived from human eIF4B around the phosphorylation site of serine 422 (T-G-SP-E-S).
Positive control
Human colon carcinoma tissue; HepG2 and HUVEC cell extracts; A549 cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab78916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
ELISA
ELISA: 1/40000ELISA: 1/40000
IHC-P: 1/50 - 1/100.
ICC/IF: 1/500 - 1/1000.
IP: Use at an assay dependent concentration.
Target
Relevance
eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).
Cellular localization
Cytoplasmic
Alternative names
- EIF 4B antibody
- Eukaryotic initiation factor 4B antibody
- Eukaryotic translation initiation factor 4B antibody
- PRO1843 antibody
Database links
- Entrez Gene: 1975 Human
- Entrez Gene: 75705 Mouse
- Omim: 603928 Human
- SwissProt: P23588 Human
- SwissProt: Q8BGD9 Mouse
- Unigene: 648394 Human
Anti-eIF4B antibody images:
Immunocytochemistry/ Immunofluorescence - eIF4B antibody (ab78916)
ab78916 at 1/500 dilution staining eIF4B in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4B antibody (ab78916)
ab78916 at 1/50 dilution staining eIF4B in human colon carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising peptide.
Western blot - eIF4B antibody (ab78916)
All lanes : Anti-eIF4B antibody (ab78916) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HUVEC cell extract
Lane 3 : HepG2 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 69 kDa
Observed band size : 69 kDa
Western blot - eIF4B antibody (ab78916)
Anti-eIF4B antibody (ab78916) at 1/500 dilution
Secondary
HRP conjugated donkey anti rabbit polyclonal at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 69 kDa
Observed band size : 69 kDa
Exposure time : 8 minutes
This image is courtesy of an anonymous Abreview
Immunoprecipitation - eIF4B antibody (ab78916)
ab78916 used in Immunprecipitation.Total protein inpute is 100µg whole cell lysate prepared from HeLa cells. Protein A/G was used in the immunoprecipitation step. ab78916 used at 8µg/mg lysate. Goat-anti human eif4b is used as primary, while the secondary is donkey anti-goat 680LT conjugated to IRDye 700DX, 1/3000 dilution.
Image courtesy of an anonymous Abreview.
References for Anti-eIF4B antibody (ab78916)
ab78916 has not yet been referenced specifically in any publications.
Publishing research using ab78916? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"