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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Anti-eIF4B (phospho S422) antibody (ab59300)
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab59300? Please let us know so that we can cite the reference in this datasheet
ab59300 has been referenced in 1 publications.
- Fromm-Dornieden C et al. Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells. BMC Mol Biol 13:9 (2012). WB. PubMed: 22436005
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - eIF4B (phospho S422) antibody (ab59300)
All lanes : Anti-eIF4B (phospho S422) antibody (ab59300) at 1/500 dilution
Lane 1 : extracts from NIH/3T3cells
Lane 2 : extracts from NIH/3T3cells with immunizing peptide
Predicted band size : 69 kDa
Observed band size : 69 kDa
Immunohistochemistry (Paraffin-embedded sections) - eIF4B (phospho S422) antibody (ab59300)
Ab59300 at 1/50 dilution staining human colon carcinoma without (left) and with (right) immunizing peptide; paraffin embedded.
Immunocytochemistry/ Immunofluorescence-eIF4B (phospho S422) antibody(ab59300)
ICC/IF image of ab59300 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59300, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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