|
|
|
|
Western blot - eIF4B (phospho S422) antibody (ab59300)
(enlarge)
|
|
Immunohistochemistry (Paraffin-embedded sections) - eIF4B (phospho S422) antibody (ab59300)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence-eIF4B (phospho S422) antibody(ab59300)
(enlarge)
|
|
|
|
|
Product Name
|
eIF4B (phospho S422) antibody
|
| |
See all eIF4B antibodies (7)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to eIF4B (phospho S422)
|
|
Immunogen
|
Synthetic phosphopeptide derived from human eIF4B around the phosphorylation site of serine 422 (T-G-SP-E-S).
|
|
Reacts with
(species key)
|
Hu, Ms, Rat
|
|
Specificity
|
Detects endogenous levels of eIF4B only when phosphorylated at serine 422.
|
|
Tested applications
(see key)
|
ELISA, ICC/IF, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/40000.
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Human colon carcinoma tissue, extracts from NIH/3T3cells.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
|
|
Relevance
|
eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).
|
|
|
eIF4B (phospho S422) antibody (ab59300) |
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Purification notes
|
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this eIF4B (phospho S422) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for eIF4B (phospho S422) antibody images, references, products related to ab59300 and other tools.
|
| |
eIF4B (phospho S422) antibody images:
|
 Western blot - eIF4B (phospho S422) antibody (ab59300)
All lanes : eIF4B (phospho S422) antibody (ab59300) at 1/500 dilution
Lane 1 : extracts from NIH/3T3cells
Lane 2 : extracts from NIH/3T3cells with immunizing peptide
Predicted band size : 69 kDa
Observed band size : 69 kDa
|
| |
Immunohistochemistry (Paraffin-embedded sections) - eIF4B (phospho S422) antibody (ab59300)
Ab59300 at 1/50 dilution staining human colon carcinoma without (left) and with (right) immunizing peptide; paraffin embedded.
|
| |
Immunocytochemistry/ Immunofluorescence-eIF4B (phospho S422) antibody(ab59300)
ICC/IF image of ab59300 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59300, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
|
| |
| |
|
Search PubMed (MEDLINE) for references to eIF4B
|
| |
eIF4B (phospho S422) antibody - more information
|
Customer reviews (feedback) regarding eIF4B (phospho S422) antibody |
Customer FAQs regarding eIF4B (phospho S422) antibody |
Protocols for eIF4B (phospho S422) antibody |
Price and availability of products related to eIF4B (phospho S422) antibody |
| |
eIF4B (phospho S422) antibody - related products:
|
|
|
| |
eIF4B (phospho S422) antibody - other tools:
|
Contact Abcam with a Technical Enquiry about eIF4B (phospho S422) antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
