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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-eIF4B (phospho S422) antibody
See all eIF4B products (7) ...
Description
Rabbit polyclonal to eIF4B (phospho S422)
Specificity
Detects endogenous levels of eIF4B only when phosphorylated at serine 422.
Tested applications
ELISA, IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from human eIF4B around the phosphorylation site of serine 422 (T-G-SP-E-S).
Positive control
Human colon carcinoma tissue, extracts from NIH/3T3cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/40000ELISA: 1/40000
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000.Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
ICC/IF: Use a concentration of 1 - 5 µg/ml.
Target
Relevance
eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).
Cellular localization
Cytoplasmic
Alternative names
- EIF 4B antibody
- EIF-4B antibody
- Eukaryotic initiation factor 4B antibody
see all
Database links
- Entrez Gene: 1975 Human
- Entrez Gene: 75705 Mouse
- Entrez Gene: 300253 Rat
- Omim: 603928 Human
- SwissProt: P23588 Human
- SwissProt: Q8BGD9 Mouse
- SwissProt: Q5RKG9 Rat
- Unigene: 648394 Human
see all
Anti-eIF4B (phospho S422) antibody images:
Western blot - eIF4B (phospho S422) antibody (ab59300)
All lanes : Anti-eIF4B (phospho S422) antibody (ab59300) at 1/500 dilution
Lane 1 : extracts from NIH/3T3cells
Lane 2 : extracts from NIH/3T3cells with immunizing peptide
Predicted band size : 69 kDa
Observed band size : 69 kDa
Immunohistochemistry (Paraffin-embedded sections) - eIF4B (phospho S422) antibody (ab59300)
Ab59300 at 1/50 dilution staining human colon carcinoma without (left) and with (right) immunizing peptide; paraffin embedded.
Immunocytochemistry/ Immunofluorescence-eIF4B (phospho S422) antibody(ab59300)
ICC/IF image of ab59300 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59300, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-eIF4B (phospho S422) antibody (ab59300)
ab59300 has not yet been referenced specifically in any publications.
Publishing research using ab59300? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"