|
|
|
|
Western blot - eIF4B (phospho422) antibody (ab71057)
(enlarge)
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4B (phospho422) antibody (ab71057)
(enlarge)
|
|
|
|
|
Product Name
|
eIF4B (phospho S422) antibody
|
| |
See all eIF4B antibodies (7)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to eIF4B (phospho S422)
|
|
Immunogen
|
KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S422 of human eIF4B.
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
ELISA, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/10 - 1/50.
WB: 1/50 - 1/100. Detects a band of approximately 79 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
lysate from transfected 293 cells; Human breast carcinoma tissue.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
|
|
Relevance
|
eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
The antibody was purified through a protein A column, followed by peptide affinity purification.
|
|
Form
|
Liquid
|
|
Concentration
|
0.250 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this eIF4B (phospho S422) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for eIF4B (phospho S422) antibody images, references, products related to ab71057 and other tools.
|
| |
eIF4B (phospho S422) antibody images:
|
 Western blot - eIF4B (phospho422) antibody (ab71057)
All lanes : eIF4B (phospho S422) antibody (ab71057) at 1/50 dilution
Lane 1 : 293 cell lysate, non transfected
Lane 2 : 293 cell lysate, transiently transfected with the eIF4B gene
Lysates/proteins at 2 µg per lane.
Observed band size : 79 kDa (why is the actual band size different from the predicted?)
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4B (phospho422) antibody (ab71057)
ab71057, at a dilution of 1/50, staining eIF4B in formalin fixed, paraffin embedded human breast carcinoma tissue (peroxidase conjugated secondary antibody, DAB staining).
|
| |
| |
|
Search PubMed (MEDLINE) for references to eIF4B
|
| |
eIF4B (phospho S422) antibody - more information
|
Customer reviews (feedback) regarding eIF4B (phospho S422) antibody |
Customer FAQs regarding eIF4B (phospho S422) antibody |
Protocols for eIF4B (phospho S422) antibody |
Price and availability of products related to eIF4B (phospho S422) antibody |
| |
eIF4B (phospho S422) antibody - related products:
|
|
|
| |
eIF4B (phospho S422) antibody - other tools:
|
Contact Abcam with a Technical Enquiry about eIF4B (phospho S422) antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
