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Anti-eIF4B antibody (ab71057)

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Overview

Product name

Anti-eIF4B antibody
See all eIF4B products (9) ...

Description

Rabbit polyclonal to eIF4B

Tested applications

IHC-P, WB, ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

KLH-conjugated synthetic peptide corresponding to amino acid residues surrounding S422 of human eIF4B.

Positive control

lysate from transfected 293 cells; Human breast carcinoma tissue.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was purified through a protein A column, followed by peptide affinity purification.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

  • Western blot - eIF4B antibody (ab71057)Western blot - eIF4B antibody (ab71057) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4B antibody (ab71057)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4B antibody (ab71057) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab71057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    IHC-P

     IHC-P: 1/10 - 1/50.

    IHC-P: 1/10 - 1/50.

  • ShowHide1 Image

    WB

     WB: 1/50 - 1/100. Detect...Read more →

    WB: 1/50 - 1/100. Detects a band of approximately 79 kDa.

  • ELISA

     ELISA: 1/1000.

    ELISA: 1/1000.

Target

Relevance

eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).

Cellular localization

Cytoplasmic

Alternative names

  • EIF 4B antibody
  • Eukaryotic initiation factor 4B antibody
  • Eukaryotic translation initiation factor 4B antibody
  • PRO1843 antibody

Database links

Anti-eIF4B antibody images:

  Western blot - eIF4B antibody (ab71057)

Western blot - eIF4B antibody (ab71057)

All lanes : Anti-eIF4B antibody (ab71057) at 1/50 dilution

Lane 1 : 293 cell lysate, non transfected
Lane 2 : 293 cell lysate, transiently transfected with the eIF4B gene

Lysates/proteins at 2 µg per lane.


Observed band size : 79 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4B antibody (ab71057)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4B antibody (ab71057)

ab71057, at a dilution of 1/50, staining eIF4B in formalin fixed, paraffin embedded human breast carcinoma tissue (peroxidase conjugated secondary antibody, DAB staining).

References for Anti-eIF4B antibody (ab71057)

ab71057 has not yet been referenced specifically in any publications.

Publishing research using ab71057? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"