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Western blot - eIF4B (phospho S504) antibody [EP2261Y] (ab75823)
![Western blot - eIF4B (phospho S504) antibody [EP2261Y] (ab75823)](/ps/datasheet/images/ab75823wb.gif)
(enlarge)
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Product Name
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eIF4B (phospho S504) antibody [EP2261Y]
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See all eIF4B antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EP2261Y] to eIF4B (phospho S504)
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Immunogen
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A phospho specific peptide corresponding to residues surrounding S504 of human eIF4B
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab75823 detects eIF4B phosphorylated at serine 504.
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Tested applications
(see key)
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ICC, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: 1/100 - 1/250.
WB: 1/25000 - 1/50000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
Is unsuitable for Flow Cyt, IHC-P or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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293T cell lysates, untreated or treated with AP.
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Cellular localization
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Cytoplasmic
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Relevance
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eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EP2261Y
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.25% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.25M EDTA, 0.18M Sodium chloride, 50mM Tris glycine, 25mM Citric acid, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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Notes
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Produced under U.S. Patent No. 5,675,063
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this eIF4B (phospho S504) antibody [EP2261Y] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for eIF4B (phospho S504) antibody [EP2261Y] images, references, products related to ab75823 and other tools.
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eIF4B (phospho S504) antibody [EP2261Y] images:
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 Western blot - eIF4B (phospho S504) antibody [EP2261Y] (ab75823)
All lanes : eIF4B (phospho S504) antibody [EP2261Y] (ab75823) at 1/50000 dilution
Lane 1 : 293T cell lysate, untreated
Lane 2 : 293T cell lysate, treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 69 kDa
Observed band size : 69 kDa
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Search PubMed (MEDLINE) for references to eIF4B
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eIF4B (phospho S504) antibody [EP2261Y] - more information
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Customer reviews (feedback) regarding eIF4B (phospho S504) antibody [EP2261Y] |
Customer FAQs regarding eIF4B (phospho S504) antibody [EP2261Y] |
Protocols for eIF4B (phospho S504) antibody [EP2261Y] |
Price and availability of products related to eIF4B (phospho S504) antibody [EP2261Y] |
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eIF4B (phospho S504) antibody [EP2261Y] - related products:
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eIF4B (phospho S504) antibody [EP2261Y] - other tools:
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Contact Abcam with a Technical Enquiry about eIF4B (phospho S504) antibody [EP2261Y] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - eIF4B (phospho S504) antibody [EP2261Y] (ab75823)](/index.html?datasheet=75823#ab75823wb.gif)
