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Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823)

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Overview

Product name

Anti-eIF4B (phospho S504) antibody [EP2261Y]
See all eIF4B products (7) ...

Description

Rabbit monoclonal [EP2261Y] to eIF4B (phospho S504)

Specificity

ab75823 detects eIF4B phosphorylated at serine 504.

Tested applications

WB, ICCmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S504 of human eIF4B

Positive control

293T cell lysates, untreated or treated with AP.

General notes

Produced under U.S. Patent No. 5,675,063

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.87% Sodium chloride, 0.05% BSA, 0.09% Tris glycine

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP2261Y

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

  • Western blot - eIF4B (phospho S504) antibody [EP2261Y] (ab75823)Western blot - eIF4B (phospho S504) antibody [EP2261Y] (ab75823) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab75823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/25000 - 1/50000.De...Read more →

    WB: 1/25000 - 1/50000.Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).

  • ICC

     ICC: 1/100 - 1/250.

    ICC: 1/100 - 1/250.

Application notes

Is unsuitable for ,Flow Cyt,IHC-P or IP.

Target

Relevance

eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).

Cellular localization

Cytoplasmic

Alternative names

  • EIF 4B antibody
  • Eukaryotic initiation factor 4B antibody
  • Eukaryotic translation initiation factor 4B antibody
  • PRO1843 antibody

Database links

see all

Anti-eIF4B (phospho S504) antibody [EP2261Y] images:

  Western blot - eIF4B (phospho S504) antibody [EP2261Y] (ab75823)

Western blot - eIF4B (phospho S504) antibody [EP2261Y] (ab75823)

All lanes : Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823) at 1/50000 dilution

Lane 1 : 293T cell lysate, untreated
Lane 2 : 293T cell lysate, treated with AP

Lysates/proteins at 10 µg per lane.

Secondary
goat anti-rabbit HRP at 1/2000 dilution

Predicted band size : 69 kDa
Observed band size : 69 kDa

References for Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823)

ab75823 has not yet been referenced specifically in any publications.

Publishing research using ab75823? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"