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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-eIF4E antibody
See all eIF4E products (13) ...
Description
Rabbit polyclonal to eIF4E
Tested applications
IP, WB, IHC-P, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
serum with 0.02% sodium azide
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
IP: 1/1000IP: 1/1000
WB: 1/1000Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
IHC-P: 1/1000Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA
ELISA: 1/1000ELISA: 1/1000
ICC/IF: Use at an assay dependent dilution. (See Abreview.)
Target
Function
Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Sequence similarities
Belongs to the eukaryotic initiation factor 4E family.
Post-translational
modifications
Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
Target information above from: UniProt accessionP06730
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EIF4F antibody
- CBP antibody
- eIF 4F 25 kDa subunit antibody
see all
Database links
- Entrez Gene: 1977 Human
- Omim: 133440 Human
- SwissProt: P06730 Human
- Unigene: 249718 Human
Anti-eIF4E antibody images:
Western blot - eIF4E antibody (ab1126)
Anti-eIF4E antibody (ab1126) + 30ug bovine kidney extract
Predicted band size : 25 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - eIF4E antibody (ab1126)
ab1126 at 1/100 staining human epithelial cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 1 hour. An Alexa-Fluor ® 647 conjugated goat anti-rabbit IgG was used as the secondary.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4E antibody (ab1126)
IHC image of ab1126 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1126, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-eIF4E antibody (ab1126)
This product has been referenced in:
- Mittal Set al. The Ccr4a (CNOT6) and Ccr4b (CNOT6L) deadenylase subunits of the human Ccr4-Not complex contribute to the prevention of cell death and senescence. Mol Biol Cell 22:748-58 (2011).Read more (PubMed: 21233283) »
- Kaiser Cet al. Activation of cap-independent translation by variant eukaryotic initiation factor 4G in vivo. RNA 14:2170-82 (2008).Read more (PubMed: 18755839) »
See all 3 publications for this product
Publishing research using ab1126? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"