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Western blot - eIF4E antibody (ab1126)
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Immunocytochemistry/ Immunofluorescence - eIF4E antibody (ab1126)
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Product Name
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eIF4E antibody
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See all eIF4E antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to eIF4E
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Immunogen
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Recombinant full length protein (Human).
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Reacts with
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Hu
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Tested applications
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Immunoprecipitation, ELISA and Western blotting: use at 1/1000 with cell extracts.
Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
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Cellular localization
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Cytoplasmic
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Research areas
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Chromatin and Nuclear Signaling >> RNAi >> Eukaryotic Initiation factors (eIF's)
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Relevance
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eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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serum with 0.02% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this eIF4E antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for eIF4E antibody images, references, products related to ab1126 and other tools.
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eIF4E antibody images:
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 Immunocytochemistry/ Immunofluorescence - eIF4E antibody (ab1126)
ab1126 at 1/100 staining human epithelial cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 1 hour. An Alexa-Fluor ® 647 conjugated goat anti-rabbit IgG was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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References for eIF4E antibody (ab1126)
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This product has been used in: (two most recent references)
Kaiser C et al. Activation of cap-independent translation by variant eukaryotic initiation factor 4G in vivo. RNA 14:2170-82 (2008). PubMed: 18755839
Muthumani K et al. The HIV-1 Vpr and glucocorticoid receptor complex is a gain-of-function interaction that prevents the nuclear localization of PARP-1. Nat Cell Biol 8:170-9 (2006). PubMed: 16429131
If you publish research using ab1126 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to eIF4E
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eIF4E antibody - more information
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Customer reviews (feedback) regarding eIF4E antibody |
Customer FAQs regarding eIF4E antibody |
Protocols for eIF4E antibody |
Price and availability of products related to eIF4E antibody |
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eIF4E antibody - related products:
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eIF4E antibody - other tools:
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Contact Abcam with a Technical Enquiry about eIF4E antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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