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Anti-eIF4E (phospho S209) antibody [EP2151Y] (ab76256)

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Overview

Product name

Anti-eIF4E (phospho S209) antibody [EP2151Y]
See all eIF4E products (13) ...

Description

Rabbit monoclonal [EP2151Y] to eIF4E (phospho S209)

Tested applications

WB, IP, IHC-P, ICCmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide corresponding to residues surrounding Serine 209 of human eIF4E

Positive control

293 cell lysates, untreated or treated with AP; human breast carcinoma tissue.

General notes

Produced under U.S. Patent No. 5,675,063

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP2151Y

Isotype

IgG

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4E (phospho S209) antibody [EP2151Y] (ab76256) image (enlarge)

  • Western blot - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)Western blot - eIF4E (phospho S209) antibody [EP2151Y] (ab76256) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab76256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/5000 - 1/10,000.De...Read more →

    WB: 1/5000 - 1/10,000.Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

  • IP

     IP: 1/60

    IP: 1/60

  • ShowHide1 Image

    IHC-P

     IHC-P: 1/100 - 1/250.Per...Read more →

    IHC-P: 1/100 - 1/250.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ICC

     ICC: 1/500

    ICC: 1/500

Application notes

Is unsuitable for or Flow Cyt.

Target

Function

Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Sequence similarities

Belongs to the eukaryotic initiation factor 4E family.

Post-translational
modifications

Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.

Target information above from: UniProt accessionP06730 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EIF4F antibody
  • CBP antibody
  • eIF 4F 25 kDa subunit antibody
  • MGC111573 antibody
  • eIF 4E antibody
  • EIF 4F antibody
  • eIF-4E antibody
  • eIF-4F 25 kDa subunit antibody
  • eIF4E antibody
  • EIF4E1 antibody
  • EIF4EL1 antibody
  • Eukaryotic translation initiation factor 4 E antibody
  • Eukaryotic translation initiation factor 4E antibody
  • Eukaryotic translation initiation factor 4E like 1 antibody
  • IF4E_HUMAN antibody
  • Messanger RNA Cap Binding Protein eIF 4E antibody
  • mRNA cap binding protein antibody
  • mRNA cap-binding protein antibody
see all

Anti-eIF4E (phospho S209) antibody [EP2151Y] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)

ab76256 at 1/100 dilution staining eIF4E in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

  Western blot - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)

Western blot - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)

All lanes : Anti-eIF4E (phospho S209) antibody [EP2151Y] (ab76256) at 1/10000 dilution

Lane 1 : 293 cell lysates, untreated
Lane 2 : 293 cell lysates, treated with AP

Lysates/proteins at 10 µg per lane.


Predicted band size : 25 kDa
Observed band size : 25 kDa

References for Anti-eIF4E (phospho S209) antibody [EP2151Y] (ab76256)

This product has been referenced in:

  • Mockett BGet al. Calcium/Calmodulin-Dependent Protein Kinase II Mediates Group I Metabotropic Glutamate Receptor-Dependent Protein Synthesis and Long-Term Depression in Rat Hippocampus. J Neurosci 31:7380-91 (2011). WB; Rat.Read more (PubMed: 21593322) »

See 1 publication for this product

Publishing research using ab76256? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"