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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)
(enlarge)


Western blot - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)
Western blot - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)
(enlarge)


Product Name 

eIF4E (phospho S209) antibody [EP2151Y]

 

See all eIF4E antibodies (10)...

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP2151Y] to eIF4E (phospho S209)

Immunogen 

A synthetic peptide corresponding to residues surrounding Serine 209 of human eIF4E

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ICC, IHC-P, IP, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ICC: 1/500.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: 1/60.
WB: 1/5000 - 1/10,000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Is unsuitable for Flow Cyt.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

293 cell lysates, untreated or treated with AP; human breast carcinoma tissue.

Cellular localization 

Cytoplasmic

Research areas 

Chromatin and Nuclear Signaling >> RNAi >> Eukaryotic Initiation factors (eIF's)
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

Relevance 

eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

1977    (Human)
13684    (Mouse)
117045    (Rat)

Omim

    

133440    (Human)

SwissProt

    

P06730    (Human)
P63073    (Mouse)
Q8C470    (Mouse)
Q3TK95    (Mouse)
P63074    (Rat)

Unigene

    

249718    (Human)
3941    (Mouse)
11275    (Rat)

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP2151Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

Notes 

Produced under U.S. Patent No. 5,675,063

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this eIF4E (phospho S209) antibody [EP2151Y] is on this datasheet. But please do contact us if you would like any reassurance!


See below for eIF4E (phospho S209) antibody [EP2151Y] images, references, products related to ab76256 and other tools.

 

eIF4E (phospho S209) antibody [EP2151Y] images:

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)

ab76256 at 1/100 dilution staining eIF4E in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

 

Product image  Western blot - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)

Western blot - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)

All lanes : eIF4E (phospho S209) antibody [EP2151Y] (ab76256) at 1/10000 dilution

Lane 1 : 293 cell lysates, untreated
Lane 2 : 293 cell lysates, treated with AP

Lysates/proteins at 10 µg per lane.


Predicted band size : 25 kDa
Observed band size : 25 kDa

 
 

Search PubMed (MEDLINE) for references to eIF4E

 

eIF4E (phospho S209) antibody [EP2151Y] - more information

Customer reviews (feedback) regarding eIF4E (phospho S209) antibody [EP2151Y]

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Protocols for eIF4E (phospho S209) antibody [EP2151Y]

Price and availability of products related to eIF4E (phospho S209) antibody [EP2151Y]

 

eIF4E (phospho S209) antibody [EP2151Y] - related products:

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