![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4E (phospho S209) antibody [EP2151Y] (ab76256) image](#ab76256ihc.gif)
![Western blot - eIF4E (phospho S209) antibody [EP2151Y] (ab76256) image](#ab76256wb.gif)
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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-eIF4E (phospho S209) antibody [EP2151Y]
See all eIF4E products (13) ...
Description
Rabbit monoclonal [EP2151Y] to eIF4E (phospho S209)
Tested applications
WB, IP, IHC-P, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues surrounding Serine 209 of human eIF4E
Positive control
293 cell lysates, untreated or treated with AP; human breast carcinoma tissue.
General notes
Produced under U.S. Patent No. 5,675,063
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP2151Y
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000 - 1/10,000.Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IP
IP: 1/60IP: 1/60
IHC-P: 1/100 - 1/250.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC
ICC: 1/500ICC: 1/500
Application notes
Is unsuitable for or Flow Cyt.
Target
Function
Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Sequence similarities
Belongs to the eukaryotic initiation factor 4E family.
Post-translational
modifications
Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
Target information above from: UniProt accessionP06730
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EIF4F antibody
- CBP antibody
- eIF 4F 25 kDa subunit antibody
see all
Database links
- Entrez Gene: 1977 Human
- Entrez Gene: 13684 Mouse
- Entrez Gene: 117045 Rat
- Omim: 133440 Human
- SwissProt: P06730 Human
- SwissProt: P63073 Mouse
- SwissProt: P63074 Rat
- Unigene: 249718 Human
see all
Anti-eIF4E (phospho S209) antibody [EP2151Y] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)](/ps/datasheet/Images/76/ab76256/ab76256ihc.gif)
ab76256 at 1/100 dilution staining eIF4E in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Western blot - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)
![Western blot - eIF4E (phospho S209) antibody [EP2151Y] (ab76256)](/ps/datasheet/Images/76/ab76256/ab76256wb.gif)
All lanes : Anti-eIF4E (phospho S209) antibody [EP2151Y] (ab76256) at 1/10000 dilution
Lane 1 : 293 cell lysates, untreated
Lane 2 : 293 cell lysates, treated with AP
Lysates/proteins at 10 µg per lane.
Predicted band size : 25 kDa
Observed band size : 25 kDa
References for Anti-eIF4E (phospho S209) antibody [EP2151Y] (ab76256)
This product has been referenced in:
- Mockett BGet al. Calcium/Calmodulin-Dependent Protein Kinase II Mediates Group I Metabotropic Glutamate Receptor-Dependent Protein Synthesis and Long-Term Depression in Rat Hippocampus. J Neurosci 31:7380-91 (2011). WB; Rat.Read more (PubMed: 21593322) »
See 1 publication for this product
Publishing research using ab76256? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"