eIF4E (phospho S209) antibody [EP2151Y] (ab76256)

Product Name 

eIF4E (phospho S209) antibody [EP2151Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP2151Y] to eIF4E (phospho S209)

Immunogen 

A synthetic peptide corresponding to residues surrounding Serine 209 of human eIF4E

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ICC, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC: 1/500.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: 1/60.
WB: 1/5000 - 1/10,000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Is unsuitable for Flow Cyt.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

293 cell lysates, untreated or treated with AP; human breast carcinoma tissue.

Cellular localization 

Cytoplasmic

Research areas 

Chromatin and Nuclear Signaling >> RNAi >> Eukaryotic Initiation factors (eIF's)
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

Relevance 

eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP2151Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

Notes 

Produced under U.S. Patent No. 5,675,063

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