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Western blot - eIF4E (phospho S209) antibody (ab47605)
Western blot - eIF4E (phospho S209) antibody (ab47605)
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Immunohistochemistry (Paraffin-embedded sections) - eIF4E (phospho S209) antibody (ab47605)
Immunohistochemistry (Paraffin-embedded sections) - eIF4E (phospho S209) antibody (ab47605)
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Product Name 

eIF4E (phospho S209) antibody

 

See all eIF4E antibodies (10)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to eIF4E (phospho S209)

Immunogen 

A synthesized phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody is specific for elF4E, only when phosphorylated at serine 209.

Tested applications 
(see key)

ELISA, IHC-P, WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: Extracts from NIH/3T3 cells
IHC: Human breast carcinoma tissue

Cellular localization 

Cytoplasmic

Research areas 

Chromatin and Nuclear Signaling >> RNAi >> Eukaryotic Initiation factors (eIF's)
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

Relevance 

eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.

 

Alternative names eIF4E (phospho S209) antibody (ab47605)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

1977    (Human)
13684    (Mouse)
117045    (Rat)

Omim

    

133440    (Human)

SwissProt

    

P06730    (Human)
P63073    (Mouse)
Q8C470    (Mouse)
Q3TK95    (Mouse)
P63074    (Rat)

Unigene

    

249718    (Human)
3941    (Mouse)
11275    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this eIF4E (phospho S209) antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for eIF4E (phospho S209) antibody images, references, products related to ab47605 and other tools.

 

eIF4E (phospho S209) antibody images:

Product image  Western blot - eIF4E (phospho S209) antibody (ab47605)

Western blot - eIF4E (phospho S209) antibody (ab47605)

All lanes : eIF4E (phospho S209) antibody (ab47605) at 1/500 dilution

Lane 1 : Extracts from NIH/3T3 cells, treated with FBS. No peptide.
Lane 2 : Extracts from NIH/3T3 cells, not treated with FBS. No peptide.

Lysates/proteins at 30 µg per lane.


Predicted band size : 25 kDa
Observed band size : 25 kDa

 

Product image  Immunohistochemistry (Paraffin-embedded sections) - eIF4E (phospho S209) antibody (ab47605)

Immunohistochemistry (Paraffin-embedded sections) - eIF4E (phospho S209) antibody (ab47605)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47605 at a 1/50 dilution.

 
 

Search PubMed (MEDLINE) for references to eIF4E

 

eIF4E (phospho S209) antibody - more information

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eIF4E (phospho S209) antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for eIF4E (phospho S209) antibody

ab7179

  

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

 

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