Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-eIF4E (phospho S209) antibody
See all eIF4E products (13) ...
Description
Rabbit polyclonal to eIF4E (phospho S209)
Specificity
This antibody is specific for elF4E, only when phosphorylated at serine 209.
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthesized phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T)
Positive control
WB: Extracts from NIH/3T3 cells IHC: Human breast carcinoma tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
WB: 1/500 - 1/1000.Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P: 1/50 - 1/100.
Target
Function
Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Sequence similarities
Belongs to the eukaryotic initiation factor 4E family.
Post-translational
modifications
Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
Target information above from: UniProt accessionP06730
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EIF4F antibody
- CBP antibody
- eIF 4F 25 kDa subunit antibody
see all
Database links
- Entrez Gene: 1977 Human
- Entrez Gene: 13684 Mouse
- Entrez Gene: 117045 Rat
- Omim: 133440 Human
- SwissProt: P06730 Human
- SwissProt: P63073 Mouse
- SwissProt: P63074 Rat
- Unigene: 249718 Human
see all
Anti-eIF4E (phospho S209) antibody images:
Western blot - eIF4E (phospho S209) antibody (ab47605)
All lanes : Anti-eIF4E (phospho S209) antibody (ab47605) at 1/500 dilution
Lane 1 : Extracts from NIH/3T3 cells, treated with FBS. No peptide.
Lane 2 : Extracts from NIH/3T3 cells, not treated with FBS. No peptide.
Lysates/proteins at 30 µg per lane.
Predicted band size : 25 kDa
Observed band size : 25 kDa
Immunohistochemistry (Paraffin-embedded sections) - eIF4E (phospho S209) antibody (ab47605)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47605 at a 1/50 dilution.
References for Anti-eIF4E (phospho S209) antibody (ab47605)
ab47605 has not yet been referenced specifically in any publications.
Publishing research using ab47605? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"