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Anti-eIF4E (phospho S209) antibody (ab47605)

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Overview

Product name

Anti-eIF4E (phospho S209) antibody
See all eIF4E products (13) ...

Description

Rabbit polyclonal to eIF4E (phospho S209)

Specificity

This antibody is specific for elF4E, only when phosphorylated at serine 209.

Tested applications

ELISA, WB, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthesized phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T)

Positive control

WB: Extracts from NIH/3T3 cells IHC: Human breast carcinoma tissue

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - eIF4E (phospho S209) antibody (ab47605)Western blot - eIF4E (phospho S209) antibody (ab47605) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - eIF4E (phospho S209) antibody (ab47605)Immunohistochemistry (Paraffin-embedded sections) - eIF4E (phospho S209) antibody (ab47605) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab47605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/20000

    ELISA: 1/20000

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

Target

Function

Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Sequence similarities

Belongs to the eukaryotic initiation factor 4E family.

Post-translational
modifications

Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.

Target information above from: UniProt accessionP06730 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EIF4F antibody
  • CBP antibody
  • eIF 4F 25 kDa subunit antibody
  • MGC111573 antibody
  • eIF 4E antibody
  • EIF 4F antibody
  • eIF-4E antibody
  • eIF-4F 25 kDa subunit antibody
  • eIF4E antibody
  • EIF4E1 antibody
  • EIF4EL1 antibody
  • Eukaryotic Translation Initiation Factor 4 E antibody
  • Eukaryotic translation initiation factor 4E antibody
  • Eukaryotic translation initiation factor 4E like 1 antibody
  • IF4E_HUMAN antibody
  • Messanger RNA Cap Binding Protein eIF 4E antibody
  • mRNA cap binding protein antibody
  • mRNA cap-binding protein antibody
see all

Anti-eIF4E (phospho S209) antibody images:

  Western blot - eIF4E (phospho S209) antibody (ab47605)

Western blot - eIF4E (phospho S209) antibody (ab47605)

All lanes : Anti-eIF4E (phospho S209) antibody (ab47605) at 1/500 dilution

Lane 1 : Extracts from NIH/3T3 cells, treated with FBS. No peptide.
Lane 2 : Extracts from NIH/3T3 cells, not treated with FBS. No peptide.

Lysates/proteins at 30 µg per lane.


Predicted band size : 25 kDa
Observed band size : 25 kDa

  Immunohistochemistry (Paraffin-embedded sections) - eIF4E (phospho S209) antibody (ab47605)

Immunohistochemistry (Paraffin-embedded sections) - eIF4E (phospho S209) antibody (ab47605)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47605 at a 1/50 dilution.

References for Anti-eIF4E (phospho S209) antibody (ab47605)

ab47605 has not yet been referenced specifically in any publications.

Publishing research using ab47605? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"