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Anti-eIF4EBP1 antibody [Y329] (ab32024)

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Overview

Product name

Anti-eIF4EBP1 antibody [Y329]
See all eIF4EBP1 products (21) ...

Description

Rabbit monoclonal [Y329] to eIF4EBP1

Tested applications

WB, IHC-P, ICC, Flow Cyt, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide corresponding to residues near the N-term of human eIF4EBP1 (aa16-33): PATRRVVLGDGVQLPPGDC

Positive control

293 cell lysate and human colon carcinoma

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Primary antibody notes

Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while, the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction

Clonality

Monoclonal

Clone number

Y329

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> RNAi >> Eukaryotic Initiation factors (eIF's)
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

  • Western blot - eIF4EBP1 antibody [Y329] (ab32024)Western blot - eIF4EBP1 antibody [Y329] (ab32024) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - eIF4EBP1 antibody [Y329] (ab32024)Immunohistochemistry (Paraffin-embedded sections) - eIF4EBP1 antibody [Y329] (ab32024) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/5000 - 1/10000. De...Read more →

    WB: 1/5000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/100 - 1/250.

    IHC-P: 1/100 - 1/250.

  • ICC

     ICC: 1/100 - 1/250.

    ICC: 1/100 - 1/250.

  • Flow Cyt

     Flow Cyt: 1/20.

    Flow Cyt: 1/20.

  • IP

     IP: 1/50.

    IP: 1/50.

Target

Function

Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

Sequence similarities

Belongs to the eIF4E-binding protein family.

Post-translational
modifications

Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.

Target information above from: UniProt accessionQ13541 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 4E BP1 antibody
  • 4E-BP1 antibody
  • 4EBP1 antibody
  • 4EBP1 antibody
  • 4EBP1_HUMAN antibody
  • BP 1 antibody
  • BP1 antibody
  • eIF4E binding protein 1 antibody
  • eIF4E-binding protein 1 antibody
  • Eif4ebp1 antibody
  • Eukaryotic translation initiation factor 4E binding protein 1 antibody
  • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
  • MGC4316 antibody
  • PHAS I antibody
  • PHAS-I antibody
  • PHASI antibody
  • Phosphorylated heat and acid stable protein regulated by insulin 1 antibody
  • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
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Database links

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Anti-eIF4EBP1 antibody [Y329] images:

  Western blot - eIF4EBP1 antibody [Y329] (ab32024)

Western blot - eIF4EBP1 antibody [Y329] (ab32024)

Anti-eIF4EBP1 antibody [Y329] (ab32024) at 1/10000 dilution + 293 cell lysate

Predicted band size : 13 kDa
Observed band size : 15 - 20 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - eIF4EBP1 antibody [Y329] (ab32024)

Immunohistochemistry (Paraffin-embedded sections) - eIF4EBP1 antibody [Y329] (ab32024)

ab32024, at a 1/100 dilution, staining human eIF4EBP1 in colon carcinoma by Immunohistochemistry, Paraffin embedded tissue

References for Anti-eIF4EBP1 antibody [Y329] (ab32024)

This product has been referenced in:

  • Silver N  et al. Activation of mTOR coincides with autophagy during ligation-induced atrophy in the rat submandibular gland. Cell Death Dis 1: (2010). WB; Rat.Read more (PubMed: 20890458) »

See 1 publication for this product

Publishing research using ab32024? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"