![Western blot - eIF4EBP1 antibody [Y330] (ab32130) image](#ab32130-1.JPG)
![Immunohistochemistry (Paraffin-embedded sections) - eIF4EBP1 antibody [Y330] (ab32130) image](#ab32130-2.JPG)
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Product name
Anti-eIF4EBP1 antibody [Y330]
See all eIF4EBP1 products (21) ...
Description
Rabbit monoclonal [Y330] to eIF4EBP1
Specificity
ab32130 recognises 4E-BP1 (eIF4E-binding protein) also known as PHAS.
Tested applications
WB, IHC-P, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues near the C-term of human4E-BP1
Positive control
K562 cell lysate and human prostate carcinoma
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
Y330
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Detects a band of approximately 15-20 kDa (predicted molecular weight: 12 kDa).
IHC-P: 1/50 - 1/100.
Flow Cyt
Flow Cyt: 1/20.Flow Cyt: 1/20.
IP
IP: 1/50.IP: 1/50.
Application notes
Is unsuitable for or ICC.
Target
Function
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Sequence similarities
Belongs to the eIF4E-binding protein family.
Post-translational
modifications
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
Target information above from: UniProt accessionQ13541
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 4E BP1 antibody
- 4E-BP1 antibody
- 4EBP1 antibody
see all
Database links
- Entrez Gene: 1978 Human
- Omim: 602223 Human
- SwissProt: Q13541 Human
- Unigene: 411641 Human
Anti-eIF4EBP1 antibody [Y330] images:
Western blot - eIF4EBP1 antibody [Y330] (ab32130)
![Western blot - eIF4EBP1 antibody [Y330] (ab32130)](/ps/datasheet/Images/32/ab32130/ab32130-1.JPG)
Anti-eIF4EBP1 antibody [Y330] (ab32130) at 1/1000 dilution + K562 cell lysate
Predicted band size : 12 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - eIF4EBP1 antibody [Y330] (ab32130)
![Immunohistochemistry (Paraffin-embedded sections) - eIF4EBP1 antibody [Y330] (ab32130)](/ps/datasheet/Images/32/ab32130/ab32130-2.JPG)
ab32130, staining human prostate carcinoma by immunohistochemistry, Paraffin embedded tissue
References for Anti-eIF4EBP1 antibody [Y330] (ab32130)
ab32130 has not yet been referenced specifically in any publications.
Publishing research using ab32130? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"