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ab2606 has been referenced in 5 publications.
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Predicted band size : 13 kDa
Ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100µg of RINm5F insulinoma cell lysate after exposure to high (lane 1) and low (lane 2) concentrations of leucine.
Anti-eIF4EBP1 antibody (ab2606) at 1/500 dilution + Lysate prepared from human HT1080 cell line at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 13 kDa
Observed band size : 20,22,25 kDa (why is the actual band size different from the predicted?)
Exposure time : 12 minutes
This image is a courtesy of Anonymous Abreview
Anti-eIF4EBP1 antibody (ab2606) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 13 kDa
Observed band size : 22,25 kDa (why is the actual band size different from the predicted?)
Exposure time : 12 minutes
This image is a courtesy of Anonymous Abreview
All lanes : Anti-eIF4EBP1 antibody (ab2606) at 1/1000 dilution
Lane 1 :
Lane 2 :
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
2
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