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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Anti-eIF4EBP1 antibody
See all eIF4EBP1 products (20) ...
Rabbit polyclonal to eIF4EBP1
This antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1.
EMSA, WBmore details
Reacts with
Mouse, Rat, Human, Pig
Synthetic peptide (Human) - which represents a portion of human eIF-4EBP1 encoded in part by exons 2and 3.
RINm5F insulinoma cell lysate
Liquid
Store at +4°C.
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration information loading...
Affinity purified using the immunising peptideimmobilized on solid support.
Polyclonal
IgG
Our Abpromise guarantee covers the use of ab2606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EMSA: Use at an assay dependent dilution. (PubMed: 17074885)
WB: 1/1000 - 1/6000.Detects a band of approximately 20-25 kDa (predicted molecular weight: 13 kDa).
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Belongs to the eIF4E-binding protein family.
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
Target information above from: UniProt accessionQ13541
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - eIF4EBP1 antibody (ab2606)

Predicted band size : 13 kDa
Ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100µg of RINm5F insulinoma cell lysate after exposure to high (lane 1) and low (lane 2) concentrations of leucine.
Western blot - eIF4EBP1 antibody (ab2606)

Anti-eIF4EBP1 antibody (ab2606) at 1/500 dilution + Lysate prepared from human HT1080 cell line at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 13 kDa
Observed band size : 20,22,25 kDa (why is the actual band size different from the predicted?)
Exposure time : 12 minutes
This image is a courtesy of Anonymous Abreview
Western blot - eIF4EBP1 antibody (ab2606)

Anti-eIF4EBP1 antibody (ab2606) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 13 kDa
Observed band size : 22,25 kDa (why is the actual band size different from the predicted?)
Exposure time : 12 minutes
This image is a courtesy of Anonymous Abreview
This product has been referenced in:
See all 5 publications for this product
Publishing research using ab2606? Please let us know so that we can cite the reference in this datasheet
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Predicted band size : 13 kDa
Ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100µg of RINm5F insulinoma cell lysate after exposure to high (lane 1) and low (lane 2) concentrations of leucine.

Anti-eIF4EBP1 antibody (ab2606) at 1/500 dilution + Lysate prepared from human HT1080 cell line at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 13 kDa
Observed band size : 20,22,25 kDa (why is the actual band size different from the predicted?)
Exposure time : 12 minutes
This image is a courtesy of Anonymous Abreview

Anti-eIF4EBP1 antibody (ab2606) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 13 kDa
Observed band size : 22,25 kDa (why is the actual band size different from the predicted?)
Exposure time : 12 minutes
This image is a courtesy of Anonymous Abreview
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