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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-eIF4EBP1 antibody
See all eIF4EBP1 products (20) ...
Description
Rabbit polyclonal to eIF4EBP1
Specificity
This antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1.
Tested applications
EMSA, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Pig
Immunogen
Synthetic peptide (Human) - which represents a portion of human eIF-4EBP1 encoded in part by exons 2and 3.
Positive control
RINm5F insulinoma cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purification notes
Affinity purified using the immunising peptideimmobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EMSA: Use at an assay dependent dilution. (PubMed: 17074885)
WB: 1/1000 - 1/6000.Detects a band of approximately 20-25 kDa (predicted molecular weight: 13 kDa).
Target
Function
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Sequence similarities
Belongs to the eIF4E-binding protein family.
Post-translational
modifications
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
Target information above from: UniProt accessionQ13541
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 4E BP1 antibody
- 4E-BP1 antibody
- 4EBP1 antibody
see all
Database links
- Entrez Gene: 1978 Human
- Entrez Gene: 13685 Mouse
- Entrez Gene: 397633 Pig
- Entrez Gene: 116636 Rat
- Omim: 602223 Human
- SwissProt: Q13541 Human
- SwissProt: Q60876 Mouse
- SwissProt: Q62622 Rat
see all
Anti-eIF4EBP1 antibody images:
Western blot - eIF4EBP1 antibody (ab2606)
Predicted band size : 13 kDa
Ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100µg of RINm5F insulinoma cell lysate after exposure to high (lane 1) and low (lane 2) concentrations of leucine.
Western blot - eIF4EBP1 antibody (ab2606)
Anti-eIF4EBP1 antibody (ab2606) at 1/500 dilution + Lysate prepared from human HT1080 cell line at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 13 kDa
Observed band size : 20,22,25 kDa (why is the actual band size different from the predicted?)
Exposure time : 12 minutes
This image is a courtesy of Anonymous Abreview
Western blot - eIF4EBP1 antibody (ab2606)
Anti-eIF4EBP1 antibody (ab2606) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 13 kDa
Observed band size : 22,25 kDa (why is the actual band size different from the predicted?)
Exposure time : 12 minutes
This image is a courtesy of Anonymous Abreview
References for Anti-eIF4EBP1 antibody (ab2606)
This product has been referenced in:
- Jacobson BAet al. Activated 4E-BP1 represses tumourigenesis and IGF-I-mediated activation of the eIF4F complex in mesothelioma. Br J Cancer 101:424-31 (2009). WB; Human.Read more (PubMed: 19603014) »
- Zhang Let al. Individual overexpression of five subunits of human translation initiation factor eIF3 promotes malignant transformation of immortal fibroblast cells. J Biol Chem 282:5790-800 (2007).Read more (PubMed: 17170115) »
See all 5 publications for this product
Publishing research using ab2606? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"