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Western blot - eIF4EBP1 antibody (ab2606)
(enlarge)
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Product Name
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eIF4EBP1 antibody
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See all eIF4EBP1 antibodies (18)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to eIF4EBP1
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Immunogen
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Synthetic peptide (Human) - which represents a portion of human eIF-4EBP1 encoded in part by exons 2and 3.
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Reacts with
(species key)
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Hu, Ms, Rat, Pig
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Specificity
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This antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1.
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Tested applications
(see key)
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EMSA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000 - 1/6000. Detects bands of approximately 20-25 kDa (predicted molecular weight: 13 kDa).
EMSA: Use at an assay dependent dilution from (PubMed: 17074885).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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RINm5F insulinoma cell lysate
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Cellular localization
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Cytoplasm
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Research areas
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Chromatin and Nuclear Signaling >> RNAi >> Eukaryotic Initiation factors (eIF's)
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Relevance
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eIF4EBP1 (eukaryotic translation Initiation Factor 4E Binding Protein 1) recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of mRNA secondary structures.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Storage buffer
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Tris-citrate/phosphate, pH 7-8, 0.1% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Affinity purified using the immunising peptideimmobilized on solid support.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this eIF4EBP1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for eIF4EBP1 antibody images, references, products related to ab2606 and other tools.
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eIF4EBP1 antibody images:
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 Western blot - eIF4EBP1 antibody (ab2606)
Predicted band size : 13 kDa
Ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100µg of RINm5F insulinoma cell lysate after exposure to high (lane 1) and low (lane 2) concentrations of leucine.
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References for eIF4EBP1 antibody (ab2606)
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This product has been used in: (two most recent references)
Jacobson BA et al. Activated 4E-BP1 represses tumourigenesis and IGF-I-mediated activation of the eIF4F complex in mesothelioma. Br J Cancer 101:424-31 (2009). WB; Human. PubMed: 19603014
Zhang L et al. Individual overexpression of five subunits of human translation initiation factor eIF3 promotes malignant transformation of immortal fibroblast cells. J Biol Chem 282:5790-800 (2007). PubMed: 17170115
See all 5 publication references for this product.
If you publish research using ab2606 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to eIF4EBP1
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eIF4EBP1 antibody - more information
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Customer reviews (feedback) regarding eIF4EBP1 antibody |
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eIF4EBP1 antibody - related products:
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eIF4EBP1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about eIF4EBP1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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