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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-eIF4EBP1 antibody
See all eIF4EBP1 products (20) ...
Description
Rabbit polyclonal to eIF4EBP1
Specificity
ab47428 recognises endogenous levels of total eIF4EBP1 protein. This antibody may cross-react with 4E-BP2 and 4E-BP3.
Tested applications
ELISA, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic non phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of threonine 36 (S-T-TP-P-G).
Positive control
Human breast carcinoma tissue; Extracts from EGF treated MDA-MB-435 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000.Detects a band of approximately 20 kDa (predicted molecular weight: 12 kDa).
Target
Function
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Sequence similarities
Belongs to the eIF4E-binding protein family.
Post-translational
modifications
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
Target information above from: UniProt accessionQ13541
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 4E BP1 antibody
- 4E-BP1 antibody
- 4EBP1 antibody
see all
Database links
- Entrez Gene: 1978 Human
- Entrez Gene: 13685 Mouse
- Entrez Gene: 116636 Rat
- Omim: 602223 Human
- SwissProt: Q13541 Human
- SwissProt: Q60876 Mouse
- SwissProt: Q62622 Rat
- Unigene: 411641 Human
see all
Anti-eIF4EBP1 antibody images:
Western blot - eIF4EBP1 antibody (ab47428)
All lanes : Anti-eIF4EBP1 antibody (ab47428) at 1/500 dilution
Lane 1 : EGF treated MDA-MB-435 cell extracts
Lane 2 : EGF treated MDA-MB-435 cells extracts incubated with immunizing peptide.
Predicted band size : 12 kDa
Immunohistochemistry (Paraffin-embedded sections) - eIF4EBP1 antibody (ab47428)
Immunohistochemical analysis of eIF4EBP1 expression in paraffin embedded human breast carcinoma tissue using 1/50 ab47428. Left: untreated sample. Right: sample treated with immunising peptide (negative control).
References for Anti-eIF4EBP1 antibody (ab47428)
ab47428 has not yet been referenced specifically in any publications.
Publishing research using ab47428? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"