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Immunohistochemistry (Paraffin-embedded sections) - eIF4EBP1 (phospho T36) antibody (ab47365)
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Western blot - eIF4EBP1 (phospho T36) antibody (ab47365)
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Immunocytochemistry/ Immunofluorescence-eIF4EBP1 (phospho T36) antibody(ab47365)
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Product Name
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eIF4EBP1 (phospho T36) antibody
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See all eIF4EBP1 antibodies (18)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to eIF4EBP1 (phospho T36)
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Immunogen
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The antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-TP-P-G).
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab47365 detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
ICC/IF: Use at a concentration of 1-5 µg/ml.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 13 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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MDA-MB-435 cell extracts; Human breast carcinoma tissue
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Cellular localization
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Cytoplasm
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Research areas
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Chromatin and Nuclear Signaling >> RNAi >> Eukaryotic Initiation factors (eIF's)
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Relevance
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eIF4EBP1 (eukaryotic translation Initiation Factor 4E Binding Protein 1) recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of mRNA secondary structures.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this eIF4EBP1 (phospho T36) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for eIF4EBP1 (phospho T36) antibody images, references, products related to ab47365 and other tools.
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eIF4EBP1 (phospho T36) antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - eIF4EBP1 (phospho T36) antibody (ab47365)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47365. Left image without immunising peptide treatment; right image with immunising peptide treatment.
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Western blot - eIF4EBP1 (phospho T36) antibody (ab47365)
All lanes : eIF4EBP1 (phospho T36) antibody (ab47365)
Lane 1 : MDA-MB-435 cells,
treated with EGF (200 ng/ml, 30min)
Lane 2 : MDA-MB-435 cells, untreated
Predicted band size : 13 kDa
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Immunocytochemistry/ Immunofluorescence-eIF4EBP1 (phospho T36) antibody(ab47365)
ICC/IF image of ab47365 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47365, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to eIF4EBP1
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eIF4EBP1 (phospho T36) antibody - more information
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Customer reviews (feedback) regarding eIF4EBP1 (phospho T36) antibody |
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eIF4EBP1 (phospho T36) antibody - related products:
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eIF4EBP1 (phospho T36) antibody - other tools:
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