Anti-eIF4EBP1 (phospho T36) antibody (ab47365)
Key features and details
- Rabbit polyclonal to eIF4EBP1 (phospho T36)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4EBP1 (phospho T36) antibody
See all eIF4EBP1 primary antibodies -
Description
Rabbit polyclonal to eIF4EBP1 (phospho T36) -
Host species
Rabbit -
Specificity
ab47365 detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human eIF4EBP1 aa 1-100 (phospho T36).
Database link: Q13541 -
Positive control
- MDA-MB-435 cell extracts; Human breast carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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WB |
1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 13 kDa).
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
WB
1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 13 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. -
Sequence similarities
Belongs to the eIF4E-binding protein family. -
Post-translational
modificationsPhosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 1978 Human
- Entrez Gene: 13685 Mouse
- Entrez Gene: 116636 Rat
- Omim: 602223 Human
- SwissProt: Q13541 Human
- SwissProt: Q60876 Mouse
- SwissProt: Q62622 Rat
- Unigene: 411641 Human
see all -
Alternative names
- 4E-BP1 antibody
- 4EBP1 antibody
- 4EBP1_HUMAN antibody
see all
Images
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All lanes : Anti-eIF4EBP1 (phospho T36) antibody (ab47365)
Lane 1 : MDA-MB-435 cells,
treated with EGF (200 ng/ml, 30min)
Lane 2 : MDA-MB-435 cells, untreated
Predicted band size: 13 kDa -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47365. Left image without immunising peptide treatment; right image with immunising peptide treatment.
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ICC/IF image of ab47365 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47365, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab47365 has been referenced in 7 publications.
- Wang L et al. Role of p75 neurotrophin receptor in neuronal autophagy in intracerebral hemorrhage in rats through the mTOR signaling pathway. Cell Cycle 19:376-389 (2020). PubMed: 31924125
- Liu J et al. Valsartan ameliorates high glucose-induced peritoneal ?brosis by blocking mTORC1 signaling. Exp Biol Med (Maywood) 245:983-993 (2020). PubMed: 32408765
- Lai WS & Ding YL GNG7 silencing promotes the proliferation and differentiation of placental cytotrophoblasts in preeclampsia rats through activation of the mTOR signaling pathway. Int J Mol Med 43:1939-1950 (2019). PubMed: 30864685
- Liu J et al. Mammalian Target of Rapamycin Complex 1 Activation Disrupts the Low-Density Lipoprotein Receptor Pathway: A Novel Mechanism for Extracellular Matrix Accumulation in Human Peritoneal Mesothelial Cells. Am J Nephrol 48:357-368 (2018). PubMed: 30423569
- Lu J et al. MicroRNA-218-5p as a Potential Target for the Treatment of Human Osteoarthritis. Mol Ther 25:2676-2688 (2017). PubMed: 28919376
- Jiang Z et al. Blocking mammalian target of rapamycin alleviates bone cancer pain and morphine tolerance via µ-opioid receptor. Int J Cancer 138:2013-20 (2016). WB ; Rat . PubMed: 26566757
- Wang S et al. Predictive value of phosphorylated mammalian target of rapamycin for disease-free survival in breast cancer patients receiving neoadjuvant chemotherapy. Oncol Lett 8:2642-2648 (2014). PubMed: 25364442