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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab27792 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - eIF4EBP1 (phospho T46) antibody (ab27792)
All lanes : Anti-eIF4EBP1 (phospho T46) antibody (ab27792) at 1/1000 dilution (diluted in 3% BSA TBST buffer, incubated for two
hours at room temperature.)
Lane 1 : PDGF treated NIH3T3 cell lysate
Lane 2 : PI3K inhibitor LY2904002 treated NIH3T3 cell lysate
Lane 3 : PI3K inhibitor LY2904002 and PDGF treated NIH3T3 cell lysate
Secondary
goat F(ab)2 anti rabbit IgG
HRP conjugate
Predicted band size : 13 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
The figure shows that ab27792 can detect phosphorylated eIF4EBP1 in PDGF treated NIH3T3 cells (lane 1), but does not detect the unphosphorylated form (lanes 2 and 3: controls).
Blocking step: 3% BSA TBST buffer for one hour at room temperature.
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