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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-eIF4EBP1 (phospho T46) antibody
See all eIF4EBP1 products (20) ...
Description
Rabbit polyclonal to eIF4EBP1 (phospho T46)
Specificity
Ab27792 recognises eIF4EBP1 that is phosphorylated at threonine 46.
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic phosphopeptide derived from the region of human eIF4EBP1 that contains threonine 46. The sequence is conserved in human, mouse and rat.
Positive control
Human embryonic kidney (HEK293) cells stimulated with EGF or 15% serum, or NIH3T3 cells stimulated with PDGF.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide.
Constituents: 50% Glycerol, PBS (without Mg2+ and
Ca2+), 1mg/ml BSA (IgG, protease free). pH 7.3 (+/- 0.1).
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Ab27792 was purified from rabbit serum by sequential epitope specific chromatography. The antibody was negatively preadsorbed using a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non phosphorylated eIF4EBP1. The final product is generated by affinity chromatography using a eIF4EBP1 derived peptide that is phosphorylated at threonine 46.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - eIF4EBP1 (phospho T46) antibody (ab27792)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Predicted molecular weight: 13 kDa.
Target
Function
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Sequence similarities
Belongs to the eIF4E-binding protein family.
Post-translational
modifications
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
Target information above from: UniProt accessionQ13541
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 4E BP1 antibody
- 4E-BP1 antibody
- 4EBP1 antibody
see all
Database links
- Entrez Gene: 1978 Human
- Entrez Gene: 13685 Mouse
- Omim: 602223 Human
- SwissProt: Q13541 Human
- SwissProt: Q60876 Mouse
- Unigene: 411641 Human
- Unigene: 6700 Mouse
Anti-eIF4EBP1 (phospho T46) antibody images:
Western blot - eIF4EBP1 (phospho T46) antibody (ab27792)
All lanes : Anti-eIF4EBP1 (phospho T46) antibody (ab27792) at 1/1000 dilution (diluted in 3% BSA TBST buffer, incubated for two
hours at room temperature.)
Lane 1 : PDGF treated NIH3T3 cell lysate
Lane 2 : PI3K inhibitor LY2904002 treated NIH3T3 cell lysate
Lane 3 : PI3K inhibitor LY2904002 and PDGF treated NIH3T3 cell lysate
Secondary
goat F(ab)2 anti rabbit IgG
HRP conjugate
Predicted band size : 13 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
The figure shows that ab27792 can detect phosphorylated eIF4EBP1 in PDGF treated NIH3T3 cells (lane 1), but does not detect the unphosphorylated form (lanes 2 and 3: controls).
Blocking step: 3% BSA TBST buffer for one hour at room temperature.
References for Anti-eIF4EBP1 (phospho T46) antibody (ab27792)
ab27792 has not yet been referenced specifically in any publications.
Publishing research using ab27792? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"