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Western blot - eIF4EBP1 (phospho T46) antibody (ab27792)
(enlarge)
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Product Name
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eIF4EBP1 (phospho T46) antibody
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See all eIF4EBP1 antibodies (18)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to eIF4EBP1 (phospho T46)
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Immunogen
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Synthetic phosphopeptide derived from the region of human eIF4EBP1 that contains threonine 46. The sequence is conserved in human, mouse
and rat.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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Ab27792 recognises eIF4EBP1 that is phosphorylated at threonine 46.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Predicted molecular weight: 13 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human embryonic kidney (HEK293) cells stimulated with EGF or 15% serum, or NIH3T3 cells stimulated with PDGF.
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Cellular localization
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Cytoplasm
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Research areas
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Chromatin and Nuclear Signaling >> RNAi >> Eukaryotic Initiation factors (eIF's)
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Relevance
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eIF4EBP1 (eukaryotic translation Initiation Factor 4E Binding Protein 1) recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of mRNA secondary structures.
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eIF4EBP1 (phospho T46) antibody (ab27792) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide.
Constituents: 50% Glycerol, PBS (without Mg2+ and
Ca2+), 1mg/ml BSA (IgG, protease free). pH 7.3 (+/- 0.1). Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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Ab27792 was purified from rabbit serum by sequential epitope specific chromatography. The antibody was negatively preadsorbed using a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non phosphorylated eIF4EBP1. The final product is generated by affinity chromatography using a eIF4EBP1 derived peptide that is phosphorylated at threonine 46.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this eIF4EBP1 (phospho T46) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for eIF4EBP1 (phospho T46) antibody images, references, products related to ab27792 and other tools.
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eIF4EBP1 (phospho T46) antibody images:
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 Western blot - eIF4EBP1 (phospho T46) antibody (ab27792)
All lanes : eIF4EBP1 (phospho T46) antibody (ab27792) at 1/1000 dilution (diluted in 3% BSA TBST buffer, incubated for two
hours at room temperature.)
Lane 1 : PDGF treated NIH3T3 cell lysate
Lane 2 : PI3K inhibitor LY2904002 treated NIH3T3 cell lysate
Lane 3 : PI3K inhibitor LY2904002 and PDGF treated NIH3T3 cell lysate
Secondary
goat F(ab)2 anti rabbit IgG
HRP conjugate
Predicted band size : 13 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
The figure shows that ab27792 can detect phosphorylated eIF4EBP1 in PDGF treated NIH3T3 cells (lane 1), but does not detect the unphosphorylated form (lanes 2 and 3: controls).
Blocking step: 3% BSA TBST buffer for one hour at room temperature.
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Search PubMed (MEDLINE) for references to eIF4EBP1
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eIF4EBP1 (phospho T46) antibody - more information
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Customer reviews (feedback) regarding eIF4EBP1 (phospho T46) antibody |
Customer FAQs regarding eIF4EBP1 (phospho T46) antibody |
Protocols for eIF4EBP1 (phospho T46) antibody |
Price and availability of products related to eIF4EBP1 (phospho T46) antibody |
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eIF4EBP1 (phospho T46) antibody - related products:
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eIF4EBP1 (phospho T46) antibody - other tools:
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Contact Abcam with a Technical Enquiry about eIF4EBP1 (phospho T46) antibody |
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