eIF4EBP1 protein (His tag) (ab60007)

Product Name 

eIF4EBP1 protein (His tag)

Product type 

Proteins and Peptides

Peptide 

Recombinant full length eIF4EBP1 protein (Human), with N-terminal His tag, MW 18 kDa, expressed in insect sf9 cells.

Tested applications 
(see key)

SDS-PAGE

Application notes 
(see key)

Recommended dilutions
SDS-PAGE: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Chromatin and Nuclear Signaling >> RNAi >> Eukaryotic Initiation factors (eIF's)
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

Relevance 

eIF4EBP1 (eukaryotic translation Initiation Factor 4E Binding Protein 1) recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of mRNA secondary structures.

Purity 

Purified via His tag

Storage buffer 

Preservative: 150mM Imidazole
Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.2mM DTT, 0.1mM PMSF, pH 7.0

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Phosphate MSDS

Purification notes 

Purity >95% as determined by densitometry.

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

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