Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-eIF4ENIF1 antibody
See all eIF4ENIF1 products (7) ...
Description
Goat polyclonal to eIF4ENIF1
Tested applications
IHC-P, ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide: AKVISVDELEYRQ, corresponding to C terminal amino acids 972-985 of Human eIF4ENIF1.
AKVISVDELEYRQ
Positive control
HEK293 Lysate.
General notes
GenBank Accession Number – NP_062817.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/ml.
ICC/IF: Use at an assay dependent concentration. PubMed: 22090346
WB: Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 140-150 kDa.The predicted molecular weight is 108 kDa according to NP_062817, however our observation agrees with that of Dostie et al, PMID: 10856257.
Target
Function
Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
Tissue specificity
Widely expressed.
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.
Target information above from: UniProt accessionQ9NRA8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2610509L04Rik antibody
- 4E T antibody
- 4E-T antibody
see all
Database links
- Entrez Gene: 56478 Human
- Entrez Gene: 74203 Mouse
- Omim: 607445 Human
- SwissProt: Q9NRA8 Human
- SwissProt: Q9EST3 Mouse
- Unigene: 517559 Human
- Unigene: 255649 Mouse
Anti-eIF4ENIF1 antibody images:
Western blot - eIF4ENIF1 antibody (ab6034)
ab6034 at 0.25µg/ml staining approximately 140-150kDa band of EIF4ENIF1 in HEK293 Lysate by Western blot (ECL).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4ENIF1 antibody (ab6034)
ab6034 at 5ug/ml staining eIF4ENIF1 in human pancreas tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
References for Anti-eIF4ENIF1 antibody (ab6034)
This product has been referenced in:
- Marnef A et al. RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor. Mol Biol Cell 23:213-24 (2012). ICC/IF; Human.Read more (PubMed: 22090346) »
See 1 publication for this product
Publishing research using ab6034? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"