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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-eIF4GII antibody
See all eIF4GII products (2) ...
Description
Rabbit polyclonal to eIF4GII
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human eIF4GII.
(Peptide available as ab41580.)
Positive control
This antibody gave a positive signal in the following human whole cell lysates: HeLa, A431, HepG2 and U20S
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 185 kDa (predicted molecular weight: 177 kDa).
Target
Relevance
Eukaryotic translation initiation factor 4 gamma3 (eIF4GII) is a probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. It is thought to be a functional homolog of EIF4G1. It interacts with EIF4A, EIF4E, eIF3 and PABPC1. There are 2 named isoforms produced by alternative splicing.
Alternative names
- eIF-4-gamma 3 antibody
- eIF-4-gamma II antibody
- eIF-4G 3 antibody
see all
Database links
- Entrez Gene: 282221 Cow
- Entrez Gene: 8672 Human
- Omim: 603929 Human
- SwissProt: O43432 Human
- SwissProt: Q504Z1 Human
- SwissProt: Q5SVN3 Human
- SwissProt: Q5SWC3 Human
- SwissProt: Q5SWD1 Human
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Anti-eIF4GII antibody images:
Western blot - eIF4GII antibody (ab40996)
All lanes : Anti-eIF4GII antibody (ab40996) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 177 kDa
Observed band size : 185 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,150 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - eIF4GII antibody (ab40996)
ICC/IF image of ab40996 stained human MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab40996, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.
References for Anti-eIF4GII antibody (ab40996)
This product has been referenced in:
- Marcet-Palacios Met al. Granzyme B Inhibits Vaccinia Virus Production through Proteolytic Cleavage of Eukaryotic Initiation Factor 4 Gamma 3. PLoS Pathog 7:e1002447 (2011). WB; Human.Read more (PubMed: 22194691) »
See 1 publication for this product
Publishing research using ab40996? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"