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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Anti-eIF5A antibody [EP526Y] (ab32443)
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Related products
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Secondary antibodies
- Lysates
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab32443? Please let us know so that we can cite the reference in this datasheet
ab32443 has been referenced in 1 publications.
- Patel PH et al. The Drosophila deoxyhypusine hydroxylase homologue nero and its target eIF5A are required for cell growth and the regulation of autophagy. J Cell Biol 185:1181-94 (2009). WB, IHC-FoFr. PubMed: 19546244
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - eIF5A antibody [EP526Y] (ab32443)](/ps/datasheet/Images/32/ab32443/ab32443_1.jpg)
Western blot - eIF5A antibody [EP526Y] (ab32443)
Anti-eIF5A antibody [EP526Y] (ab32443) at 1/10000 dilution + Jurkat cell lysate.
Predicted band size : 18 kDa
Observed band size : 18 kDa
![Immunohistochemistry (Paraffin-embedded sections) - eIF5A antibody [EP526Y] (ab32443)](/ps/datasheet/Images/32/ab32443/ab32443_2.jpg)
Immunohistochemistry (Paraffin-embedded sections) - eIF5A antibody [EP526Y] (ab32443)
ab32443 at a 1:250 dilution staining eIF5A in human adenocarcinoma of uterus using Immunohistochemistry, Paraffin Embedded Tissue.
](/ps/datasheet/images/32/ab32443/eIF5A-Primary-antibodies-ab32443-1.jpg)
Flow Cytometry-eIF5A antibody [EP526Y](ab32443)
Overlay histogram showing Jurkat cells stained with ab32443 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32443, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
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