![Western blot - eIF5A antibody [EP526Y] (ab32443) image](#ab32443_1.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - eIF5A antibody [EP526Y] (ab32443) image](#ab32443_2.jpg)
 image](#eIF5A-Primary-antibodies-ab32443-1.jpg)
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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-eIF5A antibody [EP526Y]
See all eIF5A products (6) ...
Description
Rabbit monoclonal [EP526Y] to eIF5A
Tested applications
IHC-FoFr, WB, IHC-P, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Fruit fly (Drosophila melanogaster)
Immunogen
Synthetic peptide corresponding to residues in the N-terminus of Human eIF5A.
Positive control
Jurkat cell lysate and Adenocarcinoma of human uterus slides.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 0.87% Sodium chloride, 0.09% Tris glycine
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
EP526Y
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr
IHC-FoFr: 1/400IHC-FoFr: 1/400
WB: 1/5000 - 1/10000.Predicted molecular weight: 18 kDa.
IHC-P: 1/250 - 1/500.
Flow Cyt: 1/50 - 1/80.
IP
IP: 1/50IP: 1/50
Application notes
Is unsuitable for or ICC.
Target
Function
mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
Tissue specificity
Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).
Sequence similarities
Belongs to the eIF-5A family.
Post-translational
modifications
eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
Cellular localization
Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
Target information above from: UniProt accessionP63241
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- eIF 4D antibody
- eIF 5A 1 antibody
- EIF 5A antibody
see all
Database links
- Entrez Gene: 1984 Human
- Entrez Gene: 276770 Mouse
- Entrez Gene: 287444 Rat
- Omim: 600187 Human
- SwissProt: P63241 Human
- SwissProt: Q7L7L3 Human
- SwissProt: Q7Z4L1 Human
- SwissProt: Q8WXQ9 Human
see all
Anti-eIF5A antibody [EP526Y] images:
Western blot - eIF5A antibody [EP526Y] (ab32443)
![Western blot - eIF5A antibody [EP526Y] (ab32443)](/ps/datasheet/Images/32/ab32443/ab32443_1.jpg)
Anti-eIF5A antibody [EP526Y] (ab32443) at 1/10000 dilution + Jurkat cell lysate.
Predicted band size : 18 kDa
Observed band size : 18 kDa
Immunohistochemistry (Paraffin-embedded sections) - eIF5A antibody [EP526Y] (ab32443)
![Immunohistochemistry (Paraffin-embedded sections) - eIF5A antibody [EP526Y] (ab32443)](/ps/datasheet/Images/32/ab32443/ab32443_2.jpg)
ab32443 at a 1:250 dilution staining eIF5A in human adenocarcinoma of uterus using Immunohistochemistry, Paraffin Embedded Tissue.
Flow Cytometry-eIF5A antibody [EP526Y](ab32443)
](/ps/datasheet/images/32/ab32443/eIF5A-Primary-antibodies-ab32443-1.jpg)
Overlay histogram showing Jurkat cells stained with ab32443 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32443, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
References for Anti-eIF5A antibody [EP526Y] (ab32443)
This product has been referenced in:
- Patel PHet al. The Drosophila deoxyhypusine hydroxylase homologue nero and its target eIF5A are required for cell growth and the regulation of autophagy. J Cell Biol 185:1181-94 (2009). WB, IHC-FoFr.Read more (PubMed: 19546244) »
See 1 publication for this product
Publishing research using ab32443? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"