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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - eIF5A antibody [EP527Y] (ab32407)](/ps/datasheet/Images/32/ab32407/ab32407_1.JPG)
Western blot - eIF5A antibody [EP527Y] (ab32407)
Anti-eIF5A antibody [EP527Y] (ab32407) at 1/1000 dilution + Jurkat cell lysate
Predicted band size : 18 kDa
Observed band size : 18 kDa
![Immunohistochemistry (Paraffin-embedded sections) - eIF5A antibody [EP527Y] (ab32407)](/ps/datasheet/Images/32/ab32407/ab32407_2.JPG)
Immunohistochemistry (Paraffin-embedded sections) - eIF5A antibody [EP527Y] (ab32407)
Ab32407, at a dilution of 1/250, staining eIF5A in paraffin embedded human urinary bladder carcinoma tissue sections by Immunohistochemistry.
](/ps/datasheet/images/32/ab32407/eIF5A-Primary-antibodies-ab32407-2.jpg)
Flow Cytometry-Anti-eIF5A antibody [EP527Y](ab32407)
Overlay histogram showing Jurkat cells stained with ab32407 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32407, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
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