eIF5A antibody [EP527Y] (ab32407)

Product Name 

eIF5A antibody [EP527Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP527Y] to eIF5A

Immunogen 

A synthetic peptide corresponding to residues near the C terminus of Human eIF5A.

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

Flow Cyt, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/80.
IHC-P: 1/250 - 1/500.
WB: 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).

Is unsuitable for ICC or IP.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: Jurkat cell lysates.
IHC-P: human urinary bladder carcinoma.

Cellular localization 

Cytoplasm. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus, nuclear pore complex.
Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.

Research areas 

Chromatin and Nuclear Signaling >> RNAi >> Eukaryotic Initiation factors (eIF's)
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

Relevance 

The human eukaryotic translation initiation factor 5A (eIF5A) is an 18 kDa protein. eIF5A precursor [(ec-eIF-5A(lys)] is the only cellular protein known to contain a specific lysine residue which is transformed into the unique amino acid hypusine [N-(4-amino-2-hydroxybutyl)-lysine] by a series of post translational reactions. eIF5A promotes the formation of the first peptide bond during the initial stage of protein synthesis.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP527Y

Isotype 

IgG

Storage buffer 

Preservative: 0.25% Sodium Azide
Constituents: 40% Glycerol, 0.5% BSA, 0.18M Sodium chloride, 50mM Tris glycine, 25mM EDTA, 25mM Citric acid. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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