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Overview

  • Product nameAnti-eNOS (phospho S1177) antibodySee all eNOS primary antibodies ...
  • Description
    Rabbit polyclonal to eNOS (phospho S1177)
  • SpecificityThis antibody detects endogenous levels of eNOS only when phosphorylated at serine 1176
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human eNOS (phospho S1177).

  • Positive controlHeLa cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab51038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
ELISA ELISA: 1/20000.

Target

  • FunctionProduces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
  • Tissue specificityPlatelets, placenta, liver and kidney.
  • Sequence similaritiesBelongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Cellular localizationCell membrane. Membrane > caveola. Cytoplasm > cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.

    • Target information above from: UniProt accession P29474 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      cNOS antibodyConstitutive NOS antibodyEC NOS antibody
      EC-NOS antibodyecNOS antibodyEndothelial nitric oxidase synthase antibodyEndothelial nitric oxide synthase antibodyEndothelial nitric oxide synthase 3 antibodyEndothelial NOS antibodyeNOS antibodyNitric oxide synthase 3 (endothelial cell) antibodyNitric oxide synthase 3 antibodyNitric oxide synthase 3 endothelial cell antibodyNitric oxide synthase endothelial antibodyNitric oxide synthase, endothelial antibodyNOS 3 antibodyNOS III antibodyNOS type III antibodyNOS3 antibodyNOS3_HUMAN antibodyNOSIII antibody
    see all

Anti-eNOS (phospho S1177) antibody images

  • Western blot - eNOS (phospho S1176) antibody (ab51038)
    Western blot - eNOS (phospho S1176) antibody (ab51038)
    All lanes : Anti-eNOS (phospho S1177) antibody (ab51038) at 1/500 dilution

    Lane 1 : HeLa cell extract treated with Insulin (0.01 U/ml for 15min), with the immunising peptide
    Lane 2 : HeLa cell extract treated with Insulin (0.01 U/ml for 15min), without the immunising peptide


    Predicted band size : 133 kDa
    Observed band size : 133 kDa

References for Anti-eNOS (phospho S1177) antibody (ab51038)

This product has been referenced in:
  • Cheang WS  et al. Endothelial nitric oxide synthase enhancer reduces oxidative stress and restores endothelial function in db/db mice. Cardiovasc Res 92:267-75 (2011). Read more (PubMed: 21875904) »

See 1 Publication for this product

Publishing research using ab51038 ? Please let us know so that we can cite the reference in this datasheet

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I would like to ask regarding  positive controls of ab51038. on datasheet, HeLa cell extract. Could you recommend different positive controls of ab51038? Our user has difficult to testing using HeLa cell. I look forward to hearing from you. Thank you.

Thank you for your patience. I managed to locate a publication that used endothelia from aorta of rats for an eNOS (phospho S1176) experiment. Therefore, this can also be used as a positive control for ab51038. Please see the Materials and Methods...

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We then need to place an order of an antibody against human e-nos phosphorilated on serine 1177. We found in ABCAM catalogue the antibody ab 51038, which is described to be phosphorilated in position S1176, but this should be a mistake, since position 11...

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Thank you for your enquiry. I can see from the SwissProt database entry for this protein (http://us.expasy.org/cgi-bin/niceprot.pl?P29474) that they list amino acid 1177 as being a phosphoserine. If you look at the sequence, however, they are includi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"