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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Overview
Product name
Anti-eRF3/GSPT1 antibody
See all eRF3/GSPT1 products (2) ...
Description
Rabbit polyclonal to eRF3/GSPT1
Tested applications
WB, IP, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Rabbit, Xenopus laevis, Zebrafish, a wide range of other species, Cynomolgus Monkey, Trypanosoma cruzi
Immunogen
Synthetic peptide: FETGFEKGGQTREHA conjugated to KLH by a Cysteine residue linker, corresponding to internal sequence amino acids 190-204 of Human eRF3/GSPT1
FETGFEKGGQ TREHA
Positive control
HeLa cell lysates
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1.0 µg/ml.Predicted molecular weight: 55 kDa.
IP: Use a concentration of 1 - 2 µg/ml.
ICC/IF: Use a concentration of 5 - 10 µg/ml.(Paraformadehyde fixed cells)
Target
Relevance
Translation termination in eukaryotes is governed by two proteins, belonging to the class-1 (eRF1) and class-2 (eRF3) polypeptide release factors. eRF3/GSPT1 is involved in regulation of mammalian cell growth and belongs to the GTP-binding elongation factor family (ERF3 subfamily). eRF3 catalyzes hydrolysis of GTP to GDP and inorganic phosphate in the ribosome in the absence of mRNA, tRNA, aminoacyl-tRNA and peptidyl-tRNA.
Cellular localization
Cytoplasmic
Alternative names
- 551G9.2 antibody
- Anti Eukaryotic Release Factor 3a antibody
- Anti G1 to S phase transition 1 antibody
see all
Database links
- Entrez Gene: 2935 Human
- Entrez Gene: 14852 Mouse
- Entrez Gene: 24420 Rat
- Entrez Gene: 399269 Xenopus laevis
- Entrez Gene: 325284 Zebrafish
- Omim: 139259 Human
- SwissProt: P15170 Human
- SwissProt: Q8K2E1 Mouse
see all
References for Anti-eRF3/GSPT1 antibody (ab49878)
This product has been referenced in:
- Kumar GRet al. Importin {alpha}-Mediated Nuclear Import of Cytoplasmic Poly(A) Binding Protein Occurs as a Direct Consequence of Cytoplasmic mRNA Depletion. Mol Cell Biol 31:3113-25 (2011). WB, ICC/IF; Human.Read more (PubMed: 21646427) »
- Singh Get al. A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. PLoS Biol 6:e111 (2008). IP; Human.Read more (PubMed: 18447585) »
See all 2 publications for this product
Publishing research using ab49878? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"