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Product Name
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eRF3/GSPT1 antibody
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See all eRF3/GSPT1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to eRF3/GSPT1
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Immunogen
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Synthetic peptide: FETGFEKGGQTREHA conjugated to KLH by a Cysteine residue linker, corresponding to internal sequence amino acids 190-204 of Human eRF3/GSPT1
BLAST 'FETGFEKGGQTREHA' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Many, CynMk, Rb, Try c, Xl, Zfsh
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 - 10 µg/ml. (Tested using Paraformadehyde fixed cells).
IP: Use at a concentration of 1 - 2 µg/ml.
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Predicted molecular weight: 55 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell lysates
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Cellular localization
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Cytoplasmic
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
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Relevance
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Translation termination in eukaryotes is governed by two proteins, belonging to the class-1 (eRF1) and class-2 (eRF3) polypeptide release factors. eRF3/GSPT1 is involved in regulation of mammalian cell growth and belongs to the GTP-binding elongation factor family (ERF3 subfamily). eRF3 catalyzes hydrolysis of GTP to GDP and inorganic phosphate in the ribosome in the absence of mRNA, tRNA, aminoacyl-tRNA and peptidyl-tRNA.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this eRF3/GSPT1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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References for eRF3/GSPT1 antibody (ab49878)
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This product has been used in:
Singh G et al. A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. PLoS Biol 6:e111 (2008). IP; Human. PubMed: 18447585
If you publish research using ab49878 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to eRF3/GSPT1
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eRF3/GSPT1 antibody - more information
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Contact Abcam with a Technical Enquiry about eRF3/GSPT1 antibody |
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