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Anti-eRF3/GSPT1 antibody (ab49932)

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Overview

Product name

Anti-eRF3/GSPT1 antibody
See all eRF3/GSPT1 products (2) ...

Description

Rabbit polyclonal to eRF3/GSPT1

Specificity

ab49932 recognizes human eRF3/GSPT1.

Tested applications

WB, IPmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Rabbit, Zebrafish, Cynomolgus Monkey

Immunogen

Synthetic peptide: GMVDKRTLEKYEREAKEK conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 99-116 of Human eRF3/GSPT1

GMVDKRTLEK YEREAKEK

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HeLa cell lysate

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome

Applications

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Our Abpromise guarantee covers the use of ab49932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Translation termination in eukaryotes is governed by two proteins, belonging to the class-1 (eRF1) and class-2 (eRF3) polypeptide release factors. eRF3/GSPT1 is involved in regulation of mammalian cell growth and belongs to the GTP-binding elongation factor family (ERF3 subfamily). eRF3 catalyzes hydrolysis of GTP to GDP and inorganic phosphate in the ribosome in the absence of mRNA, tRNA, aminoacyl-tRNA and peptidyl-tRNA.

Cellular localization

Cytoplasmic

Alternative names

  • 551G9.2 antibody
  • Anti Eukaryotic Release Factor 3a antibody
  • Anti G1 to S phase transition 1 antibody
  • Anti GST1, homolog of yeast antibody
  • eRF 3a antibody
  • eRF3a antibody
  • G1 to S phase transition 1 antibody
  • G1 to S phase transition protein 1 homolog antibody
  • G1 to S phase transition protein antibody
  • GSPT 1 antibody
  • GSPT1 antibody
  • GST 1 antibody
  • GST1 antibody
  • GTP binding protein GST1 HS antibody
  • Transition protein 1 antibody
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Database links

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References for Anti-eRF3/GSPT1 antibody (ab49932)

ab49932 has not yet been referenced specifically in any publications.

Publishing research using ab49932? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"